Fluorescence Polarization Immunoassay Theory and Application
The principles of fluorescence polarization were first developed by Perrin (1926). About 30 years later the technique was applied to biological system by Weber (1953), and its application to the antigen-antibody reaction was first described by Dandliker and Feigen (1961). Since then, the principles and practice of fluorescence polarization and fluorescence polarization immunoassay have been described in a number of review articles (Dandliker et al., 1964; Parker, 1973; Dandliker and de Saussure, 1970).
KeywordsRadium Streptomycin Anhydride Gentamicin Fluorescein
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- Nakajima K., Mochida H., Ueno T., Suzuki M., Kobayashi I., Matsuda I. and Miyai K., 1979, A simple, rapid method of blood thyroxine assay using a fluorescence polarization technique, Clin. Chem. Symposium (in Japanese) 18: 30–35.Google Scholar
- Parker C.W., 1973, Spectrofluometric methods, in “Hand Book of Experimental Immunology”, Weir D.M., ed., Blackwell Scientific Pub., Oxford.Google Scholar
- Schwenzer K.S. and Ahalt J.P., 1983, Automated fluorescence polarization immunoassay for monitoring streptomycin, Antimicrobial Agents & Chemotherapy, 23: 683–687.Google Scholar