Fluorescence Polarization Immunoassay Theory and Application

  • Fukuko Watanabe
  • Kiyoshi Miyai


The principles of fluorescence polarization were first developed by Perrin (1926). About 30 years later the technique was applied to biological system by Weber (1953), and its application to the antigen-antibody reaction was first described by Dandliker and Feigen (1961). Since then, the principles and practice of fluorescence polarization and fluorescence polarization immunoassay have been described in a number of review articles (Dandliker et al., 1964; Parker, 1973; Dandliker and de Saussure, 1970).


Congenital Adrenal Hyperplasia Fluorescence Polarization Fluorescent Molecule Globulin Fraction Fluorescence Polarization Immunoassay 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Dandliker W.B. and Feigen G.A., 1961, Quantification of the antigen-antibody reaction by the polarization of fluorescence, Biochem. Biophys. Res. Commun. 5: 299–304.PubMedCrossRefGoogle Scholar
  2. Dandliker W.B., Schapiro H.C., Meduski J.W., Alonso R., Feigen G.A. and Hamrick J.R., 1964, Application of fluorescence polarization to the antigen-antibody reaction, Theory and experimental method, Immunochemistry 1: 165–191.PubMedCrossRefGoogle Scholar
  3. Dandliker W.B. and de Saussure V.A., 1970, Fluorescence polarization in immunochemistry, Immunochemistry 7: 799–828.PubMedCrossRefGoogle Scholar
  4. Jolley M.E., 1981, Fluorescence polarization immunoassay for the determination of therapeutic drug levels in human plasma, J. Anal. Toxicol. 5: 236–240.PubMedGoogle Scholar
  5. Jolley M.E., Stroupe, S.D., Wang J., Panas H.N., Keegan C.L., Schmidt R.L. and Schwenzer K.S., 1981a, Fluorescence polarization immunoassay I. Monitoring aminoglycoside antibiotics in serum and plasma, Clin. Chem. 27: 1190–1197.PubMedGoogle Scholar
  6. Jolley M.E., Stroupe S.D., Schwenzer K.S., Wang C-H. J., Lu-Steffes M., Hill H.D., Popelka S.R., Holen J.T. and Kelso D.M., 1981b, Fluorescence polarization immunoassay III. An automated system for therapeutic drug determination, Clin. Chem. 27: 1575–1579.PubMedGoogle Scholar
  7. Kobayashi Y., Ogihara T., Amitani K., Watanabe F., Kiguchi T., Ninomiya I. and Kumahara Y., 1978, Enzyme immunoassay for cortisol in serum using cortisol 21-amine, Steroids 32: 137–144.PubMedCrossRefGoogle Scholar
  8. Kobayashi Y., Amitani K., Watanabe F. and Miyai K., 1979, Fluorescence polarization immunoassay for cortisol. Clin. Chim. Acta 92: 241–247.PubMedCrossRefGoogle Scholar
  9. Kobayashi Y., Miyai K., Tsubota N. and Watanabe F., 1979a, Direct fluorescence polarization immunoassay of serum cortisol, Steroids 34: 829–834.PubMedCrossRefGoogle Scholar
  10. Li T.M., Benovic J.L. and Burd J.F. 1981, Serum theophyllin determination by fluorescence polarization immunoassay utilizing an umbelliferone derivative, Anal. Biochem. 118: 102–107.PubMedCrossRefGoogle Scholar
  11. Lu-Steffes M., 1982, Fluorescence polarization immunoassay IV. Determination of phenytoin and phenobarbital in human serum and plasma, Clin. Chem. 28: 2278–2282.PubMedGoogle Scholar
  12. McGregor A.R., Prookall-Greening J.O., Landon J. and Smith D.S., 1978, Polarization fluoroimmunoassay of phenytoin, Clin. Chim. Acta 83: 161–166.PubMedCrossRefGoogle Scholar
  13. Muratsugu M. and Makino M., 1982, Evaluation of fluorescence polarization immunoassay for serum thyroxine determination, J. Clin. Chem. Clin. Biochem. 20: 567–570.PubMedGoogle Scholar
  14. Nakajima K., Mochida H., Ueno T., Suzuki M., Kobayashi I., Matsuda I. and Miyai K., 1979, A simple, rapid method of blood thyroxine assay using a fluorescence polarization technique, Clin. Chem. Symposium (in Japanese) 18: 30–35.Google Scholar
  15. Parker C.W., 1973, Spectrofluometric methods, in “Hand Book of Experimental Immunology”, Weir D.M., ed., Blackwell Scientific Pub., Oxford.Google Scholar
  16. Perrin F., 1926, Polarization de la lumiere de fluorescence. Vie moyenne de molecules dans l’etat excite, J. Phys. Radium. 7: 390–401.CrossRefGoogle Scholar
  17. Schwenzer K.S. and Ahalt J.P., 1983, Automated fluorescence polarization immunoassay for monitoring streptomycin, Antimicrobial Agents & Chemotherapy, 23: 683–687.Google Scholar
  18. Smith D.S., 1977, Enhancement fluoroimmunoassay of thyroxine, FEBS Lett. 77: 25–27.PubMedCrossRefGoogle Scholar
  19. Urios P., Cittanova N. and Jayle M-F., 1978, Immunoassay of the human chorionic gonodotropin using fluorescence polarization, FEBS Lett. 94: 54–58.PubMedCrossRefGoogle Scholar
  20. Watson R.A.A., Landon J., Shaw E.J. and Smith D.S., 1976, Polarization fluoroimmunoassay of gentamicin, Clin. Chim. Acta 73: 51–55.PubMedCrossRefGoogle Scholar
  21. Weber G., 1953, Rotational Brownian motion and polarization of the fluorescence of solutions, Adv. Protein Chem. 8: 415–459PubMedCrossRefGoogle Scholar
  22. Yamaguchi Y., Hayashi C. and Miyai K., 1982, Fluorescence polarization immunoassay for insulin preparation, Anal. Lett. 15: 731–737.CrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1988

Authors and Affiliations

  • Fukuko Watanabe
    • 1
  • Kiyoshi Miyai
    • 2
  1. 1.Clinical Chemistry LaboratoryKobe Women’s College of PharmacyKobeJapan
  2. 2.Department of Laboratory MedicineOsaka University Medical SchoolOsakaJapan

Personalised recommendations