Effects of Phenylhydrazine on Human Red Cell Membrane Aminophospholipids: A Fluorescamine Study
Phenylhydrazine was used as a peroxidative probe for studying human red cell membrane aminophospholipid peroxidation. A fluorescamine labeling method for aminophospholipids (1) was modified for direct measurement of the labeled aminophospholipids by fluorescence spectroscopy following separation of the phospholipid derivatives by thin layer chromatography. Phenylhydrazine-treated red cells had a significant decrease of phosphatidylethanolamine (PE) labeling by fluorescamine. This finding is consistent with our previous results in which we observed a significant degradation of PE associated with alterations in red cell membrane surface lipid packing (2). Further experiments indicated that the decreased labeling of PE was due to degradation of PE to lysophosphatidylethanolamine (LPE) and other oxidized lipids (CL). In seven persons studied, four degraded PE to LPE and three degraded PE to oxidized lipids other than LPE. These results indicate that individual variation may exist in the degradation of PE under oxidative conditions.
KeywordsFluorescent Spot Phospholipid Extraction Control Experimental Control Phospholipid Derivative Erythroleukaemic Cell
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