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Effects of Phenylhydrazine on Human Red Cell Membrane Aminophospholipids: A Fluorescamine Study

  • Z. Chen
  • A. Stern

Abstract

Phenylhydrazine was used as a peroxidative probe for studying human red cell membrane aminophospholipid peroxidation. A fluorescamine labeling method for aminophospholipids (1) was modified for direct measurement of the labeled aminophospholipids by fluorescence spectroscopy following separation of the phospholipid derivatives by thin layer chromatography. Phenylhydrazine-treated red cells had a significant decrease of phosphatidylethanolamine (PE) labeling by fluorescamine. This finding is consistent with our previous results in which we observed a significant degradation of PE associated with alterations in red cell membrane surface lipid packing (2). Further experiments indicated that the decreased labeling of PE was due to degradation of PE to lysophosphatidylethanolamine (LPE) and other oxidized lipids (CL). In seven persons studied, four degraded PE to LPE and three degraded PE to oxidized lipids other than LPE. These results indicate that individual variation may exist in the degradation of PE under oxidative conditions.

Keywords

Fluorescent Spot Phospholipid Extraction Control Experimental Control Phospholipid Derivative Erythroleukaemic Cell 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. 1.
    Rawyler, A., Roelofson, B., and Opden Kamp, J. A. F. The use of fluorescamine in intact Friend erythroleukaemic cells. Biochim. Biophys. Acta 769: 330–336, 1984.PubMedCrossRefGoogle Scholar
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    Arduini, A., Chen, Z., and Stern, A. Phenylhydrazine-induced changes in erythrocyte membrane surface lipid packing. Biochim. Biophys. Acta 862: 65–71, 1986.PubMedCrossRefGoogle Scholar
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    Stern, A. Red cell oxidative damage. In: “Oxidative Stress.” H. Sies, ed. Academic Press, London, 1985, pp. 331–349.Google Scholar
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    Arduini, A., and Stern, A. Spectrin degradation in intact red blood cells by Phenylhydrazine. Biochem. Pharm. 34: 4283–4289, 1985.PubMedCrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1987

Authors and Affiliations

  • Z. Chen
    • 1
  • A. Stern
    • 2
  1. 1.Cancer CenterUniversity of RochesterRochesterUSA
  2. 2.Department of PharmacologyNew York University Medical CenterNew YorkUSA

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