A Sensitive and Selective Method for Quantitative Analysis of Products of Lipid Peroxidation
We report here the development of a sensitive and specific method for measuring small amounts of lipid peroxides. The method is based on the derivatization of hydroxylated fatty acid methyl esters with fluorinated anhydrides and their analysis by gas chromatography (GC). The peroxidation products of arachidonic (20: 4) and linoleic (18: 2) acids obtained by lipoxygenase treatment have been analyzed by this method. The resulting fluoroacyl derivatives are stable, can be readily separated by capillary column GC, and can be measured at picogram levels using an electron capture detector (ECD). Moreover, since fluoroacyl moieties are incorporated exclusively into hydroxylated fatty acids, this method is well suited for the measurement of low levels of peroxidation products in the presence of much larger amounts of unmodified lipid in total lipid extracts.
KeywordsMethyl Ester Fatty Acid Methyl Ester Electron Capture Detector Total Lipid Extract Perfluorooctanoic Acid
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