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A Sensitive and Selective Method for Quantitative Analysis of Products of Lipid Peroxidation

  • M. M. Zaleska
  • D. F. Wilson

Abstract

We report here the development of a sensitive and specific method for measuring small amounts of lipid peroxides. The method is based on the derivatization of hydroxylated fatty acid methyl esters with fluorinated anhydrides and their analysis by gas chromatography (GC). The peroxidation products of arachidonic (20: 4) and linoleic (18: 2) acids obtained by lipoxygenase treatment have been analyzed by this method. The resulting fluoroacyl derivatives are stable, can be readily separated by capillary column GC, and can be measured at picogram levels using an electron capture detector (ECD). Moreover, since fluoroacyl moieties are incorporated exclusively into hydroxylated fatty acids, this method is well suited for the measurement of low levels of peroxidation products in the presence of much larger amounts of unmodified lipid in total lipid extracts.

Keywords

Methyl Ester Fatty Acid Methyl Ester Electron Capture Detector Total Lipid Extract Perfluorooctanoic Acid 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Plenum Press, New York 1987

Authors and Affiliations

  • M. M. Zaleska
    • 1
  • D. F. Wilson
    • 1
  1. 1.Department of Biochemistry and BiophysicsUniversity of PennsylvaniaPhiladelphiaUSA

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