An in Situ Method for Detection of Lipid Peroxidation Effects
An in situ method for observation of effects due to lipid peroxidation in biological samples has been developed by detecting the fluorescence of compounds formed in the process, such as aminoiminopropene (AIP) (excitation wavelength range 280–420 nm, emission wavelength range 380–500 nm), and by detecting the quenching of protein fluorescence. Measurements have been performed with a fiber optic light guide system coupled to either a readily available conventional fluorometer for routine sample inspections or a high-repetition-rate pulse laser system (1-psec pulses) and time resolved detection for superior sensitivity.
KeywordsLipid Peroxidation Fluorescence Emission Spectrum Tennessee Space Time Resolve Fluorescence Spectroscopy Pulse Laser System
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