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Generation of a Neutrophil Chemoattractant of the Lipoxygenase Pathway by Irradiated Bovine Aortic Endothelial Cells

  • A. Eldor
  • M. Cohn
  • I. Vlodavsky
  • E. Razin
  • E. Hy-Am
  • Z. Fuks
  • Y. Matzner

Abstract

We investigated the capacity of cultured bovine aortic endothelial cells (BAEC) to generate chemotactic factor(s) following ionizing radiation injury. BAEC in serum-free medium were irradiated, and the conditioned medium was assayed for chemotactic activity using normal human neutrophils in Boyden chambers. A rapid release of chemotactic activity dependent on the dose of radiation and the time between irradiation and collection of the medium was found. Damage of BAEC by freezing and thawing was not associated with the release of chemotactic activity. The chemotactic activity produced by irradiated BAEC was not destroyed by boiling or by treatment with trypsin, and its release was inhibited by pretreatment of the cells with a lipoxygenase inhibitor but not with a cyclooxygenase inhibitor. The release of chemotactic activity by vascular endothelium may account for the acute inflammatory response observed following ionizing irradiation.

Keywords

Conditioned Medium Chemotactic Factor Boyden Chamber Irradiate Cell Chemotactic Activity 
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Copyright information

© Plenum Press, New York 1987

Authors and Affiliations

  • A. Eldor
    • 1
  • M. Cohn
    • 1
  • I. Vlodavsky
    • 2
  • E. Razin
    • 3
  • E. Hy-Am
    • 1
  • Z. Fuks
    • 2
  • Y. Matzner
    • 4
  1. 1.Department of HematologyHadassah University HospitalJerusalemIsrael
  2. 2.Department of Radiation and Clinical OncologyHadassah University HospitalJerusalemIsrael
  3. 3.Department of BiochemistryHadassah-Hebrew University Medical SchoolJerusalemIsrael
  4. 4.Hematology UnitHadassah University Mount ScopusJerusalemIsrael

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