Abstract
We investigated the capacity of cultured bovine aortic endothelial cells (BAEC) to generate chemotactic factor(s) following ionizing radiation injury. BAEC in serum-free medium were irradiated, and the conditioned medium was assayed for chemotactic activity using normal human neutrophils in Boyden chambers. A rapid release of chemotactic activity dependent on the dose of radiation and the time between irradiation and collection of the medium was found. Damage of BAEC by freezing and thawing was not associated with the release of chemotactic activity. The chemotactic activity produced by irradiated BAEC was not destroyed by boiling or by treatment with trypsin, and its release was inhibited by pretreatment of the cells with a lipoxygenase inhibitor but not with a cyclooxygenase inhibitor. The release of chemotactic activity by vascular endothelium may account for the acute inflammatory response observed following ionizing irradiation.
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© 1987 Plenum Press, New York
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Eldor, A. et al. (1987). Generation of a Neutrophil Chemoattractant of the Lipoxygenase Pathway by Irradiated Bovine Aortic Endothelial Cells. In: Walden, T.L., Hughes, H.N. (eds) Prostaglandin and Lipid Metabolism in Radiation Injury. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-5457-4_24
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DOI: https://doi.org/10.1007/978-1-4684-5457-4_24
Publisher Name: Springer, Boston, MA
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