Role of Membrane Lipid Composition in Radiation- Induced Death of Mammalian Cells
There have been many speculations during the last 10 years concerning a possible role of membrane lipids in cellular radiation injury. This article addresses this question and discusses recent experiments related to this topic If lipids are involved in the mechanism of radiation-induced cell death, then the most probable molecular reactions underlying this effect are related to lipid peroxidation.
In the first part of this paper, it is shown that the naturally occurring polyunsaturated fatty acyl (PUFA) chains of phospholipids, arachidonic acid (20: 4), and docosahexanoic acid (22: 6) are the most radiosensitive lipid moieties and are damaged by peroxidative reactions. Protection by vitamin E is very efficient. In the second part, experiments are discussed in which mammalian cells in culture are modified so that more PUFA is present in the membrane phospholipids. Also the antioxidant status of the cells is manipulated. The effect of these membrane modifications on radiation-induced reproductive death is reported. The third part of this article is concerned with the role of membrane lipids and protective systems in radiation-induced interphase death.
KeywordsLipid Peroxidation Dose Rate Membrane Fatty Acid Trypan Blue Oxygen Enhancement Ratio
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