Leukotriene Synthesis by UV-Irradiated Macrophage Cell Cultures
Mouse peritoneal macrophage cells, prelabeled with 14C-arachidonic acid, were treated with ultraviolet radiation (254 nm) or the Ca2+ ionophore A23187. After incubation, leukotrienes were extracted from the cultures and were analyzed by high-performance liquid chromatography and liquid scintillation counting. Five14C peaks were observed in both the UV-irradiated and Ca2+-ionophore-treated culture extracts. Leukotriene C4 and leukotriene B4 were identified by coelution with standards. The UV-irradiated cell cultures produced twice as much LTC4 and four times as much LTB4 as did the Ca2+-ionophore-treated cell cultures.
KeywordsArachidonic Acid Macrophage Cell Arachidonic Acid Metabolism Arachidonic Acid Metabolite Calcium Ionophore A23187
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