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Leukotriene Synthesis by UV-Irradiated Macrophage Cell Cultures

  • J. E. Hardcastle
  • S. Minoui

Abstract

Mouse peritoneal macrophage cells, prelabeled with 14C-arachidonic acid, were treated with ultraviolet radiation (254 nm) or the Ca2+ ionophore A23187. After incubation, leukotrienes were extracted from the cultures and were analyzed by high-performance liquid chromatography and liquid scintillation counting. Five14C peaks were observed in both the UV-irradiated and Ca2+-ionophore-treated culture extracts. Leukotriene C4 and leukotriene B4 were identified by coelution with standards. The UV-irradiated cell cultures produced twice as much LTC4 and four times as much LTB4 as did the Ca2+-ionophore-treated cell cultures.

Keywords

Arachidonic Acid Macrophage Cell Arachidonic Acid Metabolism Arachidonic Acid Metabolite Calcium Ionophore A23187 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. 1.
    Samuelsson, B. Leukotrienes: Mediators of immediate hypersensitivity reactions and inflammation. Science 220: 568–576, 1983.PubMedCrossRefGoogle Scholar
  2. 2.
    Ford-Hutchinson, A. W. Leukotrienes: Their formation and role as inflammatory mediators. Fed. Proc. 44(1): 25–29, 1985.PubMedGoogle Scholar
  3. 3.
    Braun, G. A., and Buckner, A. C. Evidence of far ultraviolet light-mediated changes in plasma membrane structure and function. Biochem. Biophys. Acta 648: 263–266,1981.Google Scholar
  4. 4.
    DeLeo, V. A., Horlick, H., Hanson, D., Eisinger, M., and Harber, L.C. Ultraviolet radiation induces changes in membrane metabolism of human keratinocytes in culture. J. Invest. Derm. 83: 323–326, 1984.PubMedCrossRefGoogle Scholar
  5. 5.
    Scott, W. A., Pawlowski, N. C., Murray, H. W., Andreach, M., Zrike, J., and Cohn, Z. A. Regulation of arachidonic acid metabolism by macrophage activation. J. Exp. Med. 155: 1148–1160, 1982.PubMedCrossRefGoogle Scholar
  6. 6.
    Clancy, R. M., and Hugli, T. E. The extraction of leukotrienes (LTG4, LTD4, LTE4) from tissue fluids. Analyt. Biochem. 133: 30–39, 1983.PubMedCrossRefGoogle Scholar
  7. 7.
    Henke, D. C., Kouzan, S., and Eling, T. E. Analysis of leukotrienes, prostaglandins and other oxygenated metabolites of arachidonic acid by HPLC. Analyt. Biochem. 140: 87–94, 1984.PubMedCrossRefGoogle Scholar
  8. 8.
    Osborne, D. J., Peters, B. J., and Meade, C. J. The separation of leukotrienes and hydroxyeicosatetraenoic acid metabolites of arachidonic acid by HPLC. Prostaglandins 26: 817–832, 1982.CrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1987

Authors and Affiliations

  • J. E. Hardcastle
    • 1
  • S. Minoui
    • 2
  1. 1.Chemistry DepartmentTexas Woman’s UniversityDentonUSA
  2. 2.Biology DepartmentTexas Woman’s UniversityDentonUSA

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