Leukotriene Synthesis by UV-Irradiated Macrophage Cell Cultures

Hardcastle, J. E.; Minoui, S.

doi:10.1007/978-1-4684-5457-4_18

J. E. Hardcastle² &
S. Minoui³

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Abstract

Mouse peritoneal macrophage cells, prelabeled with ¹⁴C-arachidonic acid, were treated with ultraviolet radiation (254 nm) or the Ca²⁺ ionophore A23187. After incubation, leukotrienes were extracted from the cultures and were analyzed by high-performance liquid chromatography and liquid scintillation counting. Five¹⁴C peaks were observed in both the UV-irradiated and Ca²⁺-ionophore-treated culture extracts. Leukotriene C₄ and leukotriene B4 were identified by coelution with standards. The UV-irradiated cell cultures produced twice as much LTC₄ and four times as much LTB₄ as did the Ca²⁺-ionophore-treated cell cultures.

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Chemistry Department, Texas Woman’s University, Denton, Texas, 76204, USA
J. E. Hardcastle
Biology Department, Texas Woman’s University, Denton, Texas, 76204, USA
S. Minoui

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J. E. Hardcastle
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S. Minoui
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Armed Forces Radiobiology Research Institute, Bethesda, Maryland, USA
Thomas L. Walden Jr. & Haywood N. Hughes &

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Hardcastle, J.E., Minoui, S. (1987). Leukotriene Synthesis by UV-Irradiated Macrophage Cell Cultures. In: Walden, T.L., Hughes, H.N. (eds) Prostaglandin and Lipid Metabolism in Radiation Injury. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-5457-4_18

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DOI: https://doi.org/10.1007/978-1-4684-5457-4_18
Publisher Name: Springer, Boston, MA
Print ISBN: 978-1-4684-5459-8
Online ISBN: 978-1-4684-5457-4
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