Possible Involvement of Queuine in Oxidative Metabolism

  • L. Szabo
  • W. R. Farkas


The possibility that the base queuine (Q) or tRNA’s containing Q may play a role in controlling oxidative metabolism has been investigated. There was less thiobarbituric acid-reactive material in queuine-deficient (Q-) mouse liver and kidney than in (Q+) liver and kidney. (Q−) LM cells grown in culture had 53% less of the mitochondrial superoxide dismutase than did (Q+) cells. The enzymatic insertion of queuine into tRNA requires oxygen.


Oxidative Metabolism Oxygen Metabolism Mitochondrial Superoxide Conventional Mouse Mitochondrial Super Oxide Dismutase 


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  1. 1.
    Kasai, H., Ohashi, Z., Harada, F., Nishimura, S., Oppenheimer, N. J., Crain, P. F., Liehr, J. G., Von Minden, D. L., and McCloskey, J. A. Biochem. 14: 4198–4208, 1975.CrossRefGoogle Scholar
  2. 2.
    Kasai, H., Nakanishi, K., MacFarlane, R. D., Tongerson, D. F., Ohashi, Z., McCloskey, J. A., Gross, H. J., and Nishimura, S. J. Am. Chem. Soc. 98: 5044–5046, 1976.CrossRefGoogle Scholar
  3. 3.
    Katze, J. R. Nucleic Acids Res. 5: 2513–2522, 1978.PubMedCrossRefGoogle Scholar
  4. 4.
    Farkas, W. R. J. Biol. Chem. 255: 6832–6835, 1980.Google Scholar
  5. 5.
    Katze, J. R., and Farkas, W. R. Proc. Nat. Acad. Sci. (U.S.A.) 76: 3271–3275, 1979.PubMedCrossRefGoogle Scholar
  6. 6.
    Okada, N. S., Okada, N., Ohgi, T., Goto, T., and Nishimura, S. Biochem. 19: 395–400, 1980.CrossRefGoogle Scholar
  7. 7.
    Bochner, B. R., Lee, P. C., Wilson, S. W., Cutler, C. W., and Ames, B. N. Cell 37: 225–232, 1984.PubMedCrossRefGoogle Scholar
  8. 8.
    Janel, G., Michelsen, U., Nishimura, S., and Kersten, H. EMBO Journal 3: 1603–1608, 1984.PubMedGoogle Scholar
  9. 9.
    Reyniers, J. P., Pleasants, J. R., Wostmann, B. S., Katze, J. R., and Farkas, W. R. J. Biol. Chem. 256: 11591–11594, 1981.Google Scholar
  10. 10.
    Pleasants, J. R., Reddy, B. S., and Wostman, B. S. J. Nutr. 100: 498–508, 1970.PubMedGoogle Scholar
  11. 11.
    Reyniers, J. P., and Farkas, W. R. Anal. Biochem. 130: 427–430, 1983.Google Scholar
  12. 12.
    Katze, J. R., Gunduz, U., Smith, D. L., Cheng, C. S., and McCloskey, J. A. Biochem. 23: 1171–1176, 1984.CrossRefGoogle Scholar
  13. 13.
    Howes, M. K., and Farkas, W. R. J. Biol. Chem. 253: 9082–9087, 1978.Google Scholar
  14. 14.
    Misra, H. P., and Fridovich, I. J. Biol. Chem. 247: 3170–3175, 1972.Google Scholar
  15. 15.
    Matkovics, B., Novak, R., Hoang Duc Hanh, Szabo, L., Vanga, Sz. I., and Zolesna, G. Comp. Biochem. Physiol. 56B: 31–34, 1977.Google Scholar
  16. 16.
    Beers, R. F., Jr., and Sizer, I. W. J. Biol. Chem. 195: 133–140, 1952.Google Scholar
  17. 17.
    Chiu, D. T. Y., Stults, F. H., and Tappal, A. L. Biochem. et Biophys. Acta 445: 558–566, 1976.Google Scholar
  18. 18.
    Sedlack, I., and Lindsay, R. H. Analyt. Biochem. 25: 192–205, 1968.Google Scholar
  19. 19.
    Placer, Z. A., Chusham, L., and Johnson, B. C. Analyt. Biochem. 16: 359–364, 1966.Google Scholar
  20. 20.
    Patton, S., and Kurtz, G. J. Dairy Sci. 34: 669–674, 1951.CrossRefGoogle Scholar
  21. 21.
    Greim, H. Naunyn-Schmiedebergs Arch. Exp. Path. Pharmak. 266: 261–275, 1970.Google Scholar
  22. 22.
    Bradford, M. M. Anal. Biochem. 72: 248–254, 1976.Google Scholar

Copyright information

© Plenum Press, New York 1987

Authors and Affiliations

  • L. Szabo
    • 1
  • W. R. Farkas
    • 1
  1. 1.College of Veterinary Medicine and The Program in Environmental ToxicologyUniversity of TennesseeKnoxvilleUSA

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