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Uremic Toxins pp 277-280 | Cite as

Removal of Small Proteins — The Stepchild of Dialysis Therapy Protein Permeability of Different Hemofiltration Membranes Analysed by SDS-Polyacrylamide Gel Electrophoresis (SDS-Page)

  • H.-W. Birk
  • V. Wizeman
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 223)

Abstract

The kidney plays an essential role in the catabolism of small circulating proteins. Low molecular weight proteins (LMWP) with sizes smaller than that of albumin are filtered by the glomerulum and hydrolyzed in the proximal tubular cells after endocytotic absorption1,2,3. Daily filtered LMWP loads are in the range of 5 g. The renal catabolism is responsible for 30 – 80% of the plasma turnover of LMWP1. In renal failure this catabolism is decreased leading to increased LMWP plasma levels, which might contribute to the development of the uremic syndrome as many of the LMWP are biologically active components like enzymes or hormones.

Keywords

Uremic Toxin Densitometric Scanning Cellulose Triacetate Polysulfone Membrane Protein Permeability 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. 1.
    T. Maak, V. Johnson, S. T. Kan, J. Figueiredo, and D. Sigulem, Renal Filtration, Transport, and Metabolism of Low-Molecular Weight Proteins: A Review, Kidney Int. 16:251–270 (1979).CrossRefGoogle Scholar
  2. 2.
    T. Waldmann, W. Strober, and R. P. Mogielnicki, The Renal Handling of Low Molecular Weight Proteins, J. Clin. Invest. 51:2162–2174 (1972).PubMedCrossRefGoogle Scholar
  3. 3.
    F. A. Carone and D. P. Peterson, Hydrolysis and Transport of Small Peptides by the Proximal Tubule, Am. J. Physiol. 238:F151-F158 (1980).PubMedGoogle Scholar
  4. 4.
    A. Rockel, S. Abdelhamid, P. Fliegel, and D. Walb, Elimination of Low Molecular Weight Proteins with High Flux Membranes, Contr. Nephrol. 46:69–74 (1985).Google Scholar
  5. 5.
    S. Jörstad, L. C. Smeby, T.-E. Wideröe, and K. J. Berg, Transport of Uremic Toxins through Conventional Hemodialysis Membranes, Clin. Nephrol. 12:168–173 (1979).PubMedGoogle Scholar
  6. 6.
    V. Wizemann, H. G. Velcovsky, H. Bleyl, S. Brüning, and G. Schütterle, Removal of Hormones by Hemofiltration and Hemodialysis with a Highly Permeable Polysulfone Membrane, Contr. Nephrol. 46:61–68 (1985).Google Scholar
  7. 7.
    O. H. Lowry, N. J. Rosebrough, A. L. Farr, and R. J. Randall, Protein Measurement with the Folin Phenol Reagent, J. Biol. Chem. 193: 265–275 (1952).Google Scholar
  8. 8.
    M. K. Laemmli, Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4, Nature 227:680–685 (1970).PubMedCrossRefGoogle Scholar
  9. 9.
    B. R. Oakley, D. R. Kirsch, and N. R. Morris, A Simplified Ultrasensi tive Silver Stain for Detecting Proteins in Polyacrylamide Gels, Anal. Biochem. 105:361–363 (1980).PubMedCrossRefGoogle Scholar
  10. 10.
    J. Bergström and P. Fürst, Uremic Toxins, in: “Replacement of Renal Function by Dialysis”, W. Drükker, F. M. Parsons, and J. F. Maher, eds., pp. 354–390, Martinus Nijhoff, (1983).CrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1987

Authors and Affiliations

  • H.-W. Birk
    • 1
  • V. Wizeman
    • 1
  1. 1.Department of Internal MedicineJustus-Liebig-UniversityGiessenGermany

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