Identification of Two Uremic Toxins by Nuclear Magnetic Resonance and Mass Spectrometry
A few uremic toxins (UT) previously called uremic middle molecules were isolated in pure form and identified In a previous study, by using a three step chromatographic separation we had isolated ten uremic toxins and had identified two of them The first is a double conjugate of glucuronidate-o-hydroxyhippuric acid identical to Fürst’s compound The second is a glucuronic acid conjugate of o-hydroxybenzoic acid. In the present paper, we report the identification by 1H nuclear magnetic resonance and mass spectrometry of two new uremic toxins represented by peaks 2-5-2 and 2-5-5.
KeywordsNuclear Magnetic Resonance Uremic Toxin Coupling Feature Plasmatic Ultrafiltrate Positive Chemical Ionization
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