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Uremic Toxins pp 161-164 | Cite as

Isolation of an Immunosuppressive Fraction from Uremic Ultrafiltrates

  • J. Navarro
  • M. C. Grossetête
  • J. L. Touraine
  • J. Traeger
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 223)

Abstract

Cell mediated immune responses were decreased in patients with chronic renal failure (CRF) and this deficiency was shown to be mainly dependent on toxic or inhibitory serum factors. In previous study we have reported the isolation of a crude fraction from uremic sera with immunosuppressive properties 1,2. This crude fraction which has a molecular weight (MW) below 5.000 daltons, inhibits markedly the lymphocyte proliferation induced by several mitogens or by allogeneic cells1. Further attempts to purify and characterize this immunosuppressive factor are presented. The inhibitory effect of each eluate fraction on in vitro lymphocyte proliferation has been assessed at each step of purification.

Keywords

High Performance Liquid Chromatography High Performance Liquid Chromatography Lymphocyte Proliferation Peptidic Nature Normal Seron 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. 1.
    J. Navarro, J.L. Touraine, C. Corre, J. Traeger, Effet in vitro des “moyennes molecules” sur la prolifération lymphocytaire. Path. Biol. 24: 189 (1976)Google Scholar
  2. 2.
    J. Navarro, J.L. Touraine, C. Corre, J. Traeger, Prolongation of skin allograft survival and inhibition of graft versus host reaction in rodents treated with “middle molecules”. Cell. Immunol. 31: 349 (1977)PubMedCrossRefGoogle Scholar
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    J. Navarro, M.C. Grossetête, A. Defrasne, J.L. Touraine, J. Traeger, Isolation of an immunosuppressive fraction in ultrafiltrate from uremic sera. Nephron 40: 396 (1985)PubMedCrossRefGoogle Scholar
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    P. Contreras, R. Later, J. Navarro, J.L. Touraine, A.M. Freyria, J. Traeger, Molecules in the middle molecular weight range. Critical review of methods of separation from fluid of uremic patients. Nephron 32 (3): 193 (1982)PubMedCrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1987

Authors and Affiliations

  • J. Navarro
    • 1
  • M. C. Grossetête
    • 1
  • J. L. Touraine
    • 1
  • J. Traeger
    • 1
  1. 1.Inserm U.80, CNRS UA 1177UCBLyonFrance

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