The Immunosuppressive Fraction Isolated from Uremic Ultrafiltrates: Attempted Characterization of the Mechanism of Action
Since many years our attention has been focused on the isolation of an immunosuppressive fraction from the serum of uremic patients. This fraction which was obtained after a single chromatography of uremic fluids on a Sephadex. G25 column, was shown to inhibit markedly the lymphocyte proliferation induced by several mitogens and by allogeneic cells. A continuous infusion of this material in rats allowed a significant prolongation of skin allograft survival. By contrast the antibody production to sheep red blood cells was not affected by such a treatment. Furthermore, this crude fraction reduced significantly the graft-versus-host reaction induced in lethally irradiated mice injected with allogeneic spleen lymphocytes previously incubated in the presence of the uremic factor. Inhibition of cell proliferation appears to be the common denominator of all these in vitro and in vivo immunosuppressive effects.
KeywordsAllogeneic Cell Crude Fraction Uremic Solute Skin Allograft Survival Rative Spleen Cell
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- 1.J. Navarro, P. Contreras, J.L. Touraine, A.M. Freyria, R. Later, J. Traeger, Effect of "middle molecules" on immunological fonctions. Art. Organs 4 (suppl): 76 (1980)Google Scholar