The Fraction b 4-2: Isolation, Characterization and Biological Activities with Reference to Uremic Polyneuropathy
The toxic role of numerous compounds accumulated in the body fluids of uremic patients is not well established. Instead of comparing the overall chromatographic plasma pattern of uremic patients to that of healthy subjects (1), one uremic symptom, namely active recent polyneuritis, was chosen and the correlation between the evolution of symptoms and biochemical patterns as modified by hemodialysis was evaluated. This correlation was complemented by a bioassay in which the fraction isolated previously exhibits a specific in vitro activity similar to that observed in vivo. These clinical, biochemical and bioassay techniques each contribute to form a correlative approach (2). Uremic polyneuritis was chosen to test the “middle molecule hypothesis” because it was found to respond favorably to any method which increases middle molecule transfer, i.e., more frequent or more prolonged dialysis or the use of a more porous membrane (3). In six patients with progressive and complete motor nerve paraplegia due to inadequate dialysis, the rapid improvement of nervous symptoms when using a dialysis membrane highly permeable to middle molecules brought us the first clinical evidence for the validity of the hypothesis (4).
KeywordsFree Diet Uremic Toxin Action Potential Amplitude High Liquid Performance Chroma Motor Nerve Conduction Velocity
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