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The Regions of T-cell Recognition on the Extracellular Part of the α Chain of TorpedoCalifornica Acetylcholine Receptor

  • Tsuyoshi Yokoi
  • Biserka Mulac-Jericevic
  • M. Zouhair Atassi
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 225)

Abstract

A comprehensive synthetic approach was employed to identify the continuous regions of T-cell recognition on the α-chain of Torpedo californica acetylcholine receptor (AChR). Eighteen synthetic consecutive overlapping peptides, of uniform size and overlaps, that spanned the entire extracellular part (residues 1–210) of the a chain were examined for their in vitro stimulation of lymph node cells from AChR-primed C57BL/6 (H-2b), C3H/He (H-2k), SWR(H-2q) and SJL (H-2s) mice. The T-cell recognition sites (T sites) in the AChR-primed mouse strains resided within six regions on the extracellular part of the α-chain. Three of the regions recognized by T cells coincided with regions recognized by antibodies (i.e. B cells) and one of these three regions also coincided with an α-neurotoxin binding region. It is noteworthy that, in addition to sites recognized by both T and B cells, the protein has at least two sites which are recognized exclusively by T cells and to which no detectable antibody responses are directed.

Keywords

Mouse Strain Challenge Dose Extracellular Part Detectable Antibody Response Separate Genetic Control 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Plenum Press, New York 1987

Authors and Affiliations

  • Tsuyoshi Yokoi
    • 1
  • Biserka Mulac-Jericevic
    • 1
  • M. Zouhair Atassi
    • 1
  1. 1.Marrs McLean Department of BiochemistryBaylor College of MedicineHoustonUSA

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