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T-Cell Recognition and Antigen Presentation of Lysozyme

  • Garvin S. BixlerJr.
  • M. Zouhair Atassi
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 225)

Abstract

Several years ago, this laboratory introduced a comprehensive strategy for the systematic localization of all the continuous sites on a protein that are involved in B- and T-cell recognition. The strategy depends on the synthesis of consecutive overlapping peptides that together account for the entire protein chain. Using this approach, the full submolecular profile of continuous regions on hen egg lysozyme recognized by T cells (T sites) were localized. Four major T-cell recognition sites, three of which were subject to individual genetic control, were localized in the six mouse strains examined. In addition to these four continuous T sites, T-cell recognition of lysozyme also involved the three previously defined discontinuous antibody binding sites as demonstrated with lysozyme-specific longterm T cell cultures. Contrary to a long held impression, T-cell recognition, therefore, is not restricted only to sequence features, but can also be directed to protein discontinuous surface areas of high conformational dependency. More recently, we have examined in two mouse strains the proliferative response to peptides and to native protein of lymph node cells from mice primed with synthetic overlapping peptides either individually or as a mixture. It was found that the pattern of T-cell recognition observed after priming with peptides differs from that obtained when the native protein is used as the immunogen. If antigen processing proceeds via fragmentation, then only those regions containing T sites would be expected to be effective in priming for a T-cell response to the intact protein. Since this was not found to be the case, it is unlikely that fragmentation of lysozyme is a prerequisite for antigen presentation. Rather, we suggest that the critical aspects in the presentation of a protein antigen involve predominantly recognition of an intact protein.

Keywords

Synthetic Peptide Proliferative Response Peptide Mixture Intact Protein Stimulation Index 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Allen, P.M. and Unanue, E.R. (1984) J. Immunol. 132, 1077–1079.PubMedGoogle Scholar
  2. Allen, P.M., Strydom, D.J. and Unanue, E.R. (1984 Proc. Natl. Acad. Sci. USA 81, 2489–2493.PubMedCrossRefGoogle Scholar
  3. Allen, P.M., Beller, D.I., Braun, J. and Unanue, E.R. (1984b) J. Immunol. 132, 323–331.Google Scholar
  4. Atassi, M.Z. 1975) Immunochemistry 12, 423–438.PubMedCrossRefGoogle Scholar
  5. Atassi, M.Z. (1978) Immunochemistry 13, 909–936.CrossRefGoogle Scholar
  6. Atassi, M.Z. (1979) CRC Reviews in Biochemistry 6, 371–400.CrossRefGoogle Scholar
  7. Atassi, M.Z. (1982) Biochim. Biophys. Acta 704, 552–555.PubMedCrossRefGoogle Scholar
  8. Atassi, M.Z. and Lee, C.-L. (1978a) Biochem. J. 171, 419–427.Google Scholar
  9. Atassi, M.Z. and Lee, C.-L. (1978b) Biochem. J. 171, 429–434.Google Scholar
  10. Atassi, M.Z. and Smith, J.A. (1978) Immunochemistry 15, 609–610.PubMedCrossRefGoogle Scholar
  11. Atassi, M.Z., Lee, C.-L. and Pai, R.-C. (1976) Biochem. Biophys. Acta 427, 745–751.PubMedCrossRefGoogle Scholar
  12. Atassi, M.Z., Sakata, S. and Kazim, A.L. (1979) Biochem. J. 179, 327–331.PubMedGoogle Scholar
  13. Atassi, M.Z., Yokota, S., Twining, S.S., Lehmann, H. and David, C.S. (1981) Mol. Immunol. 18, 945–948.PubMedCrossRefGoogle Scholar
  14. Atassi, H., Ulrich, R.G. and Atassi, M.Z. (1987) Europ. J. Immunol., in press.Google Scholar
  15. Bixler, G.S. and Atassi, M.Z. (1983) Immunol. Comm. 12, 593–603.Google Scholar
  16. Bixler, G.S. and Atassi, M.Z. (1984a) J. Immunogen. 11, 339–353).CrossRefGoogle Scholar
  17. Bixler, G.S. and Atassi, M.Z. (1984b) J. Immunoqen. 11, 245–250.CrossRefGoogle Scholar
  18. Bixler, G.S., Yoshida, T. and Atassi, M.Z. (1984a) Expl. Clin. Immunogen. 1, 99–111.Google Scholar
  19. Bixler, G.S., Yoshida, T. and Atassi, M.Z. (1984b) J. Immunogen. 11, 327–337.CrossRefGoogle Scholar
  20. Bixler, G.S., Yoshida, T. and Atassi, M.Z. (1985) Immunology 56, 103–112.PubMedGoogle Scholar
  21. Bixler, G.S., Bean, M. and Atassi, M.Z. (1986) Biochem. J. 240, 139–146.PubMedGoogle Scholar
  22. Buchmuller, and Corradin, G. (1982) Eur. J. Immunol. 12, 412–416.PubMedCrossRefGoogle Scholar
  23. Chesnut, R.W., Colon, S.M. and Grey, H.M. (1982) 129, 2382–2388.Google Scholar
  24. Kazim, A.L. and Atassi, M.Z. (1980) 191, 261–264.Google Scholar
  25. Kazim, A.L. and Atassi, M.Z. (1982) Biochem. J. 203, 201–208.PubMedGoogle Scholar
  26. Keck, K. (1975) Nature 254, 78.PubMedCrossRefGoogle Scholar
  27. Koketsu, J. and Atassi, M.Z. (1973) Biochim. Biophys. Acta 328, 289–302.PubMedCrossRefGoogle Scholar
  28. Koketsu, J. and Atassi, M.Z. (1974) Immunochemistry 11, 1–8.PubMedCrossRefGoogle Scholar
  29. Krco, C.J., Kazim, A.L., Atassi, M.Z. and David, C.S.(1981) J. Immunogenet. 8, 315–322.PubMedCrossRefGoogle Scholar
  30. Lee, C.-L. and Atassi, M.Z. (1976) Biochem. J. 159, 89–93.PubMedGoogle Scholar
  31. Lee, C.-L. and Atassi, M.Z. (1977a) Biochim. Biophys. Acta 495, 354–368.CrossRefGoogle Scholar
  32. Lee, C.-L. and Atassi, M.Z. (1977b) Biochem. J. 167, 571–582.Google Scholar
  33. Lee, K.-C., Wong, M. and Spitzer, D. (1982) Transplantation 34, 150–152.PubMedCrossRefGoogle Scholar
  34. Lozner, E.C., Sachs, D.H. and Shearer, G.M. (1974) J. Exp. Med. 139. 1204.PubMedCrossRefGoogle Scholar
  35. Maizels, R.M., Clarke, J.A., Harvey, M.A., Miller, A. and Sercarz, E.E. (1980) Eur. J. Immunol. 10, 509–515.PubMedCrossRefGoogle Scholar
  36. Melchers, I., Rajewsry, K. and Shreffler, D.C. (1973) Eur. J. Immunol. 3, 754.PubMedCrossRefGoogle Scholar
  37. Mulac—Jericevic, B., Kurisaki, J. and Atassi, M.Z. (1987) Proc. Nat. Acad. Sci. USA, in press.Google Scholar
  38. Okuda, K., Christadoss, P.R., Twining, S., Atassi, M.Z. and David, C.S. 1978) J. Immunol. 121, 866–868.PubMedGoogle Scholar
  39. Okuda, K., Twining, S.S., David, C.S. and Atassi, M.Z. (1979a) J. Immunol. 123, 182–188.Google Scholar
  40. Okuda, K., Sakata, S., Atassi, M.Z. and David, C.S. (1979b) J. Immunogen. 6, 447–452.CrossRefGoogle Scholar
  41. Sakata, S. and Atassi, M.Z. (1980) Mol. Immunol. 17, 139–142.PubMedCrossRefGoogle Scholar
  42. Scibienski, R.J., Klingmann, V., Leung, C., Thompson, K. and Benjamini, E. (1978) (Atassi, M.Z. and Stavitsky, A.B., Eds.), Vol. 1, pp. 305–317. Plenum Press, New York.Google Scholar
  43. Semma, M., Sakata, N., Fujio, H. and Amano, T. (1981) Immunol. Letters 3, 57–61.CrossRefGoogle Scholar
  44. Shimnkevitz, R., Kappler, J., Marrack, P. and Grey, H. (1983) J. Exp. Med. 158, 303–316.CrossRefGoogle Scholar
  45. Sugimoto, M., Kohima, A., Yaginuma, K. and Egashira, Y. (1975) Japan J. Med. Sci. Biol. 28, 23–25.Google Scholar
  46. Thompson, K., Harris, M., Benjamini, E., Mitchell, G. and Noble, M. (1972) Nature N.B. 238, 20–21.PubMedCrossRefGoogle Scholar
  47. Twining, S.S., David, C.S. and Atassi, M.Z. (1981) Mol. Immunol. 18, 447–450.PubMedCrossRefGoogle Scholar
  48. Yoshioka, M., Bixler, G.S. and Atassi, M.Z. (1983) Mol. Immunol. 20, 1133–1137.PubMedCrossRefGoogle Scholar
  49. Yoshioka, N. and Atassi, M.Z. (1986) Biochem. J. 234, 441–447.PubMedGoogle Scholar
  50. Young, C.R. and Atassi, M.Z. (1982) Adv. Exp. Med. 150, 79–93.Google Scholar
  51. Young, C.R. and Atassi, M.Z. (1983) J. Immunogen. 10, 151–160.CrossRefGoogle Scholar
  52. Ziegler, K. and Unanue, E.R. (1981) J. Immunol. 127, 1869–1875.PubMedGoogle Scholar
  53. Ziegler, K. and Unanue, E.R. (1982) Proc. Natl. Acad. Sci. USA 79, 175–178.PubMedCrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1987

Authors and Affiliations

  • Garvin S. BixlerJr.
    • 1
  • M. Zouhair Atassi
    • 1
  1. 1.Verna and Marrs McLean Department of BiochemistryBaylor College of MedicineHoustonUSA

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