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Intermediates in Class II HLA Antigen Biosynthesis

  • Peter Cresswell
  • Drew N. Kelner
  • Michael S. Marks
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 225)

Abstract

It has been appreciated for a number of years that the biosynthesis and intracellular transport of class II HLA antigens is unusual compared to that of the majority of membrane glycoproteins studied. The α and β subunits which make up the mature class II antigen dimer are known to be associated with a third glycoprotein, the invariant or I chain, during their intracellular transport (Jones, et al., 1978; Machamer and Cresswell, 1982). The nature of this intracellular complex has been difficult to elucidate because of its transient nature. We have used the sodium ionophore monensin (Tartakoff, 1983) to arrest class II antigen transport in human B-lymphoblastoid cell lines (B-LCL), and have successfully isolated accumulated class II antigen biosynthetic intermediates using monoclonal antibody affinity columns (Reiner and Cresswell, 1986). This report summarizes our current understanding of the nature of these intermediates.

Keywords

Antigen Complex Invariant Chain High Molecular Weight Complex Tetrameric Species Chondroitin Sulfate Side Chain 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Plenum Press, New York 1987

Authors and Affiliations

  • Peter Cresswell
    • 1
  • Drew N. Kelner
    • 1
  • Michael S. Marks
    • 1
  1. 1.Department of Microbiology and ImmunologyDuke University Medical CenterDurhamUSA

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