Summary
A panel of “auto-reactive” T-cell hybridomas has been generated by fusing Sendai virus-primed, Peyer’s patch T cells from CBA mice with BW 51117. The hybridomas produce Il-2 in response to stimulation by Class II-positive syngeneic cells in the absence of added Sendai virus. The stimulator genes for two hybridoma clones have been mapped using recombinant mouse strains and the restriction has been confirmed by inhibition with monoclonal anti-Class II antibodies.
Hybridomas grown in serum-free medium do not respond to syngeneic cells in the absence of foetal calf serum (FCS) and the response is restored by the addition of FCS, but not bovine serum albumin (BSA), normal mouse serum or Sendai virus. The component of FCS which is required for stimulation of the hybridomas has been partially purified and characterised. Its mode of action has been investigated and it acts on the stimulator cells and not on the hybridomas.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Bretscher, P.A., 1986, How significant are “autoreactive” T cells?, Immunol. Today, 7: 301.
Denizot, F. and Golstein, P., 1982, The functional effects of foetal calf serum on mouse lymphocytes in culture, Bull. Inst. Pasteur, 80: 247.
Endres, R.O., Marrack, P. and Kappler, J.W., 1983, An IL-2 secreting T cell hybridoma that responds to a self class I histocompatibility antigen in the H-2D region, J. Immunol., 131: 1656.
Faherty, D.A., Johnson, D.R. and Zauderer, M., 1985, Origin and specificity of autoreactive T cells in antigen-induced populations, J. Exp. Med., 161: 1293.
Frangakis, M.V., Koopman, W.J., Kiyono, H., Michalek, S.M., and McGhee, J.R., 1982, An enzymatic method for preparation of dissociated murine Peyer’s patch cells enriched for macrophages, J. Immunol. Methods, 48: 33.
Huber, C., Merkenschlager, M., Gattringer, C., Royston, I., Fink, U., and Braunsteiner, H., 1982, Human autologous mixed lymphocyte reactivity is primarily specific for xenoprotein determinants absorbed to antigen-presenting cells during rosette formation with sheep erythrocytes, J. Exp. Med., 155: 1222.
Julius, M.H. and Heusser, C.H., 1986, Autoreactive or self-restricted?, Eur. J. Immunol., 16: 867.
Kawanishi, H., Ozato, K. and Strober, W., 1985, The proliferative response of cloned Peyer’s patch switch T cells to syngeneic and allogeneic stimuli, J. Immunol., 134: 3586.
Muller, I., and Kaufmann, S.H.E., 1985, Antigen-reactivity pattern of T-cell hybridomas from Mycobacterium bovis BCG-infected mice, Infect. Immun. 49: 838.
Rock, K.L. and Benacerraf, B., 1983, The role of Ia molecules in the activation of T lymphocytes. III. Antigen-specific, Ia-restricted interleukin 2-producing T cell hybridomas with detectable affinity for the restricting I-A molecule, J. Exp. Med. 157: 359.
Saito, K., Tamura, A., Narimatsu, H., Tadakuma, T., and Nagashima, M., 1986, Cloned auto-Ia-reactive T cells elicit lichen planus-like lesion in the skin of syngeneic mice, J. Immunol., 137: 2485.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1987 Plenum Press, New York
About this chapter
Cite this chapter
Pullen, A.M., Munro, A.J. (1987). “Auto-Reactive” T-Cell Hybridomas and the Role of Foetal Calf Serum. In: Atassi, M.Z. (eds) Immunobiology of Proteins and Peptides IV. Advances in Experimental Medicine and Biology, vol 225. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-5442-0_23
Download citation
DOI: https://doi.org/10.1007/978-1-4684-5442-0_23
Publisher Name: Springer, Boston, MA
Print ISBN: 978-1-4684-5444-4
Online ISBN: 978-1-4684-5442-0
eBook Packages: Springer Book Archive