Analysis of T-Cell Receptor Gamma Chain Expression in the Thymus
While much is known about the structure and function of the T-cell receptor (TCR) αβ heterodimer, information has just begun to emerge about the γ protein, the product of a third rearranging T-cell receptor gene. We describe the use of antiserum to a carboxy-terminal peptide common to several of the murine gamma chain constant regions and a monoclonal antibody to the murine T3 complex to identify products of this T-cell receptor gene family in a subpopulation of Lyt2−, L3T4− thymocytes. The gamma chain has a molecular weight of 35kD, is disulfide bonded to a 45kD partner termed delta (δ) and is associated with the T3 complex. The cells that bear this second T-cell receptor appear to represent a distinct lineage differentiating within the thymus.
KeywordsNonreducing Condition Irrelevant Peptide Fetal Thymocyte Surface Radio Digitonin Lysate
Unable to display preview. Download preview PDF.