Mycobacterial Antigens

Reagents for Tuberculin Skin Testing and Serodiagnosis of Tuberculosis
  • L. F. Affronti
Part of the Infectious Agents and Pathogenesis book series (IAPA)


Despite the fact that knowledge of the cause and cure of tuberculosis has been known for years, it still is one of the most communicable diseases in the world and remains as one of the largest single causes of disease and death today, particularly in developing nations. At least 15 million peo ple suffer from tuberculosis currently, which claims more than 3 million lives each year. This is particularly disturbing, since tuberculosis is a disease for which not only chemotherapeutic treatment is known and available but also because a standard regimen of antituberculosis drugs can be administered rather inexpensively and has been shown to be highly effective.


Mycobacterium Tuberculosis Skin Testing Migration Inhibitory Factor Tuberculosis Patient Culture Filtrate 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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  1. 1.
    Koch, R., 1882, Aetiologie der tuberculose, Berl. Klin. Wochenschr. 19:221–230.Google Scholar
  2. 2.
    von Pirquet, C., 1907, Demonstration zur tuberkulindiagnose durch hautimpfung, Berl. Klin. Wochenschr. 48:699.Google Scholar
  3. 3.
    Daniel, T. M., and Janicki, B. W., 1978, Mycobacterial antigens: Review of their isolation, chemistry and immunological properties, Microbiol. Rev. 42:84–113.Google Scholar
  4. 4.
    Seibert, F. B., 1934, The isolation and properties of the purified protein derivative of tuberculin, Am. Rev. Tuberc. 30:713–720.Google Scholar
  5. 5.
    Lind, P., 1947, Purified tuberculin, its preparation and properties, Acta Tuberc. Scand. 31:112–159.Google Scholar
  6. 6.
    Seibert, F. B., 1949, The isolation of three different proteins and two polysaccharides from tuberculin by alcohol fractionation. Their chemical and biological properties, Am, Rev. Tuberc. 59:86–101.Google Scholar
  7. 7.
    Castelnuovo, G. M., Duncan, M. E., and Bellezza, G., 1964, Mycobacterial antigens: A study of antigens in bacterial extracts and in culture filtrates of different ages, Tubercle 45:246–254.PubMedCrossRefGoogle Scholar
  8. 8.
    Lind, A., Norlin, M., and Ouchterlony, O., 1970, Studies on the precipitinogenic pattern of mycobacteria cultivated at various temperatures, Pneumologie 142:283–289.CrossRefGoogle Scholar
  9. 9.
    Magnusson, M., Kim, H. K., and Bentzon, M. W., 1963, Tuberculin production. 1. Yield of tuberculoprotein from various media, Acta Pathol. Microbiol, Scand. 58:363–375.CrossRefGoogle Scholar
  10. 10.
    Seibert, F. B., and Glenn, J. T., 1941, Tuberculin purified protein derivative. Prepa ration and analysis of a large quantity for standard, Am. Rev. Tuberc. 44:9–25.Google Scholar
  11. 11.
    Affronti, L. F., 1959, Purified protein derivatives (PPD) and other antigens prepared from atypical acid-fast bacilli and Nocardia asteroids, Am. Rev. Tuberc. 79:284–295.PubMedGoogle Scholar
  12. 12.
    Janicki, B. W., Wright, G. L., Jr., Good, R. C., and Chaparas, S. D., 1976, Comparison of antigens in sonic and pressure cell extracts of Mycobacterium tuberculosis, Infect. Immun. 14:425–437.Google Scholar
  13. 13.
    Daniel, T. M., Good, R. C., and Janicki, B. W., 1975, Immunoelectrophoresis of Mycobacterium tuberculosis antigens. Comparative analysis of cell extract and culture filtrate antigens, Am. Rev. Respir. Dis. 112:639–644.PubMedGoogle Scholar
  14. 14.
    Turcotte, R., and Des Ormeaux, Y., 1972, Influence of the age of mycobacterial cultures on the protein and carbohydrate composition of tuberculins, Can. J. Microbiol. 18:637–645.PubMedCrossRefGoogle Scholar
  15. 15.
    Wayne, L. G., and Sramek, H. A., 1979, Antigenic differences between extracts of actively replicating and synchronized resting cells of Mycobacterium tuberculosis, Infect. Immun. 24:363–370.PubMedGoogle Scholar
  16. 16.
    Janicki, B. W., Chaparas, S. D., Daniel, T. M., Kubica, G. P., Wright, G. L., Jr., and Yu, G. S., 1971, A reference system for antigens of Mycobacterium tuberculosis, Am. Rev. Respir. Dis. 104:602–604.PubMedGoogle Scholar
  17. 17.
    Axelsen, J. H., Harboe, M., Closs, O., and Godal, T., 1974, BCG antibody profiles in tuberculoid and lepromatous leprosy, Infect. Immun. 9:952–958.PubMedGoogle Scholar
  18. 18.
    Affronti, L. F., Parlett, R. C., and Cornesky, R. A., 1965, Electrophoresis on polyacrylamide gels of protein and polysaccharide fractions from Mycobacterium tuberculosis, Am. Rev. Respir. Dis. 91:1–5.PubMedGoogle Scholar
  19. 19.
    Roberts, D. B., Wright, G. L., Jr., Affronti, L. F., and Reich, M., 1972, Characterization and comparison of mycobacterial antigens by two-dimensional Immunoelectrophoresis, Infect. Immun. 6:564–573.PubMedGoogle Scholar
  20. 20.
    Chaparas, S. D., Brown, T. M., and Hyman, I. S., 1978, Antigenic relationships among species of Mycobacterium studied by fused rocket immunoelectrophoresis, Int. J. Syst. Bacteriol. 28:547–560.CrossRefGoogle Scholar
  21. 21.
    Closs, O., Harboe, M., Axelsen, N. H., Bunch-Christensen, K., and Magnusson, M., 1980, The antigens of Mycobacterium bovis strain BCG, studied by crossed Immu noelectrophoresis: A reference system., Scand. J. Immunol. 12:249–263.PubMedCrossRefGoogle Scholar
  22. 22.
    Stanford, J. L., and Beck, A., 1968, An antigenic analysis of the mycobacteria. Mycobacterium fortuitum Myco. kansasii, Myco phlei, Myco. smegmatis and Myco. tuberculosis, J. Pathol. 95: 131–139.CrossRefGoogle Scholar
  23. 23.
    Koch, R., 1891, Fortsetzung der Mitteilunger uber ein Heilmittel gegen tuberculose, Dtsch. Med. Woschenschr. 17:101–102.CrossRefGoogle Scholar
  24. 24.
    Chaparas, S. D., 1984, Immunologically based diagnostic tests with tuberculin and other mycobacterial antigens, in: The Mycobacteria A Sourcebook (G. P. Kubica and L. G. Wayne, eds.), pp. 195–220, Dekker, New York.Google Scholar
  25. 25.
    Seibert, F. B., 1928, The chemical composition of the active principle of tuberculin. XL. An improved and simplified method for making a standard undenatured tuberculin of any desired strength and a method of chemical assay, J. Biol. Chem. 78:345–362.Google Scholar
  26. 26.
    Masucci, P., and McAlpine, K. L., 1930, Biochemical studies of bacterial derivatives. X. Preparation of human tubercle bacillus protein MA-100, Proc. Soc. Exp. Biol. Med. 27:661–663.Google Scholar
  27. 27.
    Seibert, F. B., 1934, The isolation and properties of the purified protein derivative of tuberculin, Am. Rev. Tuberc. (Suppl.) 30:713–720.Google Scholar
  28. 28.
    WHO, 1952, World Health Organization Technical Report ,Ser. No. 56, Expert Committee on Biological Standardization, Fifth Report, July 1952 ,p. 6.Google Scholar
  29. 29.
    Tukey, J. W., DuFour, E. H., and Seibert, F. B., 1950, Lack of sensitization following repeated skin tests with standard tuberculin (PPD-S), Am. Rev. Tuberc. 62:77–86.PubMedGoogle Scholar
  30. 30.
    Seibert, F. B., Soto-Figueroa, E., and DuFour, E. H., 1955, Isolation, identification, and classification of proteins of tuberculin and the tubercle bacillus, Am. Rev. Tuberc. 71:704–721.PubMedGoogle Scholar
  31. 31.
    Birnbaum, S. E., and Affronti, L. F., 1968, Chemical and serological relationships between the heteropolysaccharides of Mycobacterium tuberculosis and Mycobacterium kansasii, J. Bacteriol. 95:559–564.PubMedGoogle Scholar
  32. 32.
    Birnbaum, S. E., 1968, A comparison of the immunochemistry of polysaccharides from M. tuberculosis and the atypical mycobacteria, dissertation, George Washington University, Washington, D.C.Google Scholar
  33. 33.
    Birnbaum, S. E., and Affronti, L. F., 1970, Mycobacterial polysaccharides. I. Serological and skin testing studies on mycobacterial polysaccharides, Int. Arch. Allergy Immunol. 37:88–97.CrossRefGoogle Scholar
  34. 34.
    Heilman, D. H., 1967, In vitro studies on polysaccharides of Mycobacterium tuberculosis and delayed hypersensitivity, Am. Rev. Respir. Dis. 96:198–203.PubMedGoogle Scholar
  35. 35.
    Seibert, F. B., Stacey, M., and Kent, P. W., 1949, An antigenic polysaccharide, “Polysaccharide II,” isolated from tuberculin, Biochim. Biophys. Acta 3:632–640.CrossRefGoogle Scholar
  36. 36.
    Daniel, T. M., and Affronti, L. F., 1973, Immunoelectrophoretic analysis of antigenic constituents of Seibert fractions of mycobacterial culture filtrates. Identification with the proposed United States-Japan reference nomenclature, Am. Rev. Respir. Dis. 108:1244–1248.Google Scholar
  37. 37.
    Daniel, T. M., 1984, Soluble mycobacterial antigens, in: The Mycobacteria. A Sourcebook (G. P. Kubica. and L. G. Wayne, eds.), pp. 417–465, Dekker, New York.Google Scholar
  38. 38.
    Kniker, W. T., and LaBorde, J. B., 1964, The separation of mycobacterial antigens by ion-exchange chromatography. I. Details of chromatographic and immunologic procedures and results with four strains of M. tuberculosis, Am. Rev. Respir. Dis. 89:29–40.PubMedGoogle Scholar
  39. 39.
    Kniker, W. T., 1965, The use of ion-exchange chromatography for mycobacterial antigen isolation, Am. Rev. Respir. Dis. (Suppl.) 92:19–28.Google Scholar
  40. 40.
    Someya, S., Hayashi, O. ,and Yamamura, Y., 1962, Studies on the antigenicity of tuberculin active peptide, Am. Rev. Respir. Dis. 86:542–546.PubMedGoogle Scholar
  41. 41.
    Someya, S., Koyama, K., Asami, N., Kataoka, T., and Yamamura, Y., 1966, Studies on the tuberculin reactivity of tuberculin peptide, Jpn. J. Microbiol. 10:221–227.Google Scholar
  42. 42.
    Yamamura, Y., Onoue, K., and Azuma, I., 1968, Chemical and immunological stud ies on peptides and polysaccharides from tubercle bacilli, Ann. NY Acad. Sci. 154:88–97.PubMedCrossRefGoogle Scholar
  43. 43.
    Yamamura, Y., Onoue, K., and Tahara, T., 1969, Purification and the properties of tuberculin active peptides, Z. Immunitaetsforch. Allergie Klin. Immunol. 137:171–176.Google Scholar
  44. 44.
    Kotani, S., Yanagida, I., Kato, K., and Matsuda, T., 1970, Studies on peptides, glycopeptides and antigenic polysaccharide-glycopeptide complexes isolated from an L-ll enzyme lysate of cell walls of Mycobacterium tuberculosis strain H37Rv, Biken J. 13:249–275.PubMedGoogle Scholar
  45. 45.
    Lind, A., 1965, Immunologic analysis of mycobacterial fractions, Am. Rev. Respir. Dis. (Suppl.) 92:54–62.Google Scholar
  46. 46.
    Janicki, B. W., Aron, S. A., and Raychaudhuri, A., 1972, Separation and isolation of mycobacterial antigens by continuous-flow electrophoresis, Am. Rev. Respir. Dis. 106:779–783.PubMedGoogle Scholar
  47. 47.
    Janicki, B. W., Goldstein, R. A., and Aron, S. A., 1971, Immunoelectrophoretic studies of the precipitating antibody response in tuberculosis, Am. Rev. Respir. Dis. 103:894.Google Scholar
  48. 48.
    Janicki, B. W., Aron, S. A., Schechter, G. P., and McFarland, W., 1972, Tuberculin antigens active in human lymphocyte blastogenesis, Proc. Soc. Exp. Biol. Med. 141:809–813.PubMedGoogle Scholar
  49. 49.
    Janicki, B. W., and Aron, S. A., 1973, Failure of isolated tuberculopolysaccharides to induce precipitins in the guinea pig, Annu. Mtg. Am. Soc. Microbiol. 123 (abst.).Google Scholar
  50. 50.
    Janicki, B. W., Goldstein, R. A. ,and Aron, S. A., 1971, Immunoelectrophoretic studies of the precipitating antibody response in tuberculosis, Am. Rev. Respir. Dis. 103:894 (abst.).Google Scholar
  51. 51.
    Daniel, T. M., and Wisnieski, J. J., 1970, The reaction of concanavalin A with mycobacterial culture filtrates, Am. Rev. Respir. Dis. 101:762–764.PubMedGoogle Scholar
  52. 52.
    Daniel, T. M., 1974, The purification of mycobacterial polysaccharides with concanavalin A, Am. Rev. Respir. Dis. 110:634–640.PubMedGoogle Scholar
  53. 53.
    Laguerre, M., and Turcotte, R., 1977, Use of sepharose-conjugated BCG antibodies for the purification of tuberculin-active components. Can. J. Microbiol. 23:695–700.PubMedCrossRefGoogle Scholar
  54. 54.
    Daniel, T. M., and Anderson, P. A., 1978, The isolation by immunoabsorbent affinity chromatography and physicochemical characterization of Mycobacterium tuberculosis antigen 5, Am. Rev. Respir. Dis. 117:533–539.PubMedGoogle Scholar
  55. 55.
    Daniel, T. M., Ellner, J. J., Todd, L. S., McCoy, D. W., Payne, V. D. H., Anderson, P. A., and Bhe, F. T., 1979, Immunobiology and species distribution of Mycobacterium tuberculosis antigen 5, Infect. Immun. 24:77–82.PubMedGoogle Scholar
  56. 56.
    Daniel, T. M., Balestrino, E. A., Balestrino, O. C., Davidson, P. T., Debanne, S. M., Kataria, S., Kataria, Y. P., and Scocozza, J. B., 1982, The tuberculin specificity in humans of Mycobacterium tuberculosis antigen 5, Am. Rev. Respir. Dis. 126:600–606.PubMedGoogle Scholar
  57. 57.
    Fukui, Y., and Yoneda, M., 1961, Extracellular proteins of tubercle bacilli. III. Column chromatographical purification of the proteins of a virulent strain (H37Rv) of Mycobacterium tuberculosis, Biken J. 4: 187–196.PubMedGoogle Scholar
  58. 58.
    Fukui, Y., Hirai, T., Uchida, T., and Yoneda, M., 1965, Extracellular proteins of tubercle bacilli. IV. and antigens as major extracellular protein products and as cellular components of a strain (H37Rv) of Mycobacterium tuberculosis, Biken J. 8:189–199.PubMedGoogle Scholar
  59. 59.
    Grange, J. M., 1984, The humoral immune response in tuberculosis: Its nature, biological role, and diagnostic usefulness, Adv. Tuberc. Res. 21:1–78.PubMedGoogle Scholar
  60. 60.
    Tasaka, H., Nomura, T., and Matsuo, Y., 1985, Specificity and distribution of alpha antigens of Mycobacterium avium-intracellulare, Mycobacterium scrofulaceum and related species of mycobacteria, Am. Rev. Respir. Dis. 132:173–174.PubMedGoogle Scholar
  61. 61.
    Daniel, T. M., and Ferguson, L. E., 1970, Purification and characterization of two proteins from culture filtrates of Mycobacterium tuberculosis H37Ra strain, Infect. Im-mun. 1:164–168.Google Scholar
  62. 62.
    Daniel, T. M., and Hinz, C. F., Jr., 1974, Reactivity of purified proteins and polysac-charides from Mycobacterium tuberculosis in delayed skin test and cultured lymphocytes mitogenesis assays, Infect. Immun. 9:44–47.PubMedGoogle Scholar
  63. 63.
    Daniel, T. M., and DeMuth, R. W., 1977, Immunochemical analysis of a major antigen of Mycobacterium szulgai,J. Infect. Dis. 135:778–786.PubMedCrossRefGoogle Scholar
  64. 64.
    Nagai, S., Nagasuga, T., Matsumoto, J., and Kohda, K., 1974, Isolation of tuberculin skin test reactive proteins from heated culture filtrate of Mycobacterium tuberculosis H37Rv, Am. Rev. Respir. Dis. 108:17–28.Google Scholar
  65. 65.
    Nagai, S., Matsumoto, J., and Nagasuga, T., 1981, Specific skin reactive protein from culture filtrate of Mycobacterium bovis BCG, Infect. Immun. 31:1152–1160.PubMedGoogle Scholar
  66. 66.
    Miura, K., Nagai, S., Kinomoto, M., Haga, S., Tokunaga, T., 1983, Comparative studies with various substrains of Mycobacterium bovis BCG on the production of an antigenic protein, MPB 70, Infect. Immun. 39:540–545.Google Scholar
  67. 67.
    Kuwabara, S., and Tsumita, T., 1974, Primary structure of tuberculin active protein from tubercle bacilli, Jpn. J. Exp. Med. 44:129–132.PubMedGoogle Scholar
  68. 68.
    Kuwabara, S., 1975, Purification and properties of tuberculin-active protein from Mycobacterium tuberculosis, J. Biol. Chem. 250:2556–2562.PubMedGoogle Scholar
  69. 69.
    Kuwabara, S., 1975, Amino acid sequence of tuberculin-active protein from Mycobacterium tuberculosis, J. Biol. Chem. 250:2563–2568.PubMedGoogle Scholar
  70. 70.
    Savrda, J., 1980, Synthesis and biological assays of a peptide from a tuberculin-active protein, Infect. Immun. 30:686–693.PubMedGoogle Scholar
  71. 71.
    Savrda, J., 1983, Synthesis and biological assays of peptides from a tuberculin active protein, Infect. Immun. 40:1163–1169.PubMedGoogle Scholar
  72. 72.
    Toida, I., Yamamoto, S., Takuma, S., Suzuki, T., and Hirato, M., 1985, Lack of tuberculin activity of synthetic peptides, Infect. Immun. 50:614–619.PubMedGoogle Scholar
  73. 73.
    Minden, P., Kelleher, P. J., Freed, J. H., Nielsen, L. D., Brennan, P. J., McPheron, L., and McClatchy, J. K., 1984, Immunological evaluation of a component isolated from Mycobacterium bovis BCG with a monoclonal antibody to M. bovis BCG, Infect. Immun. 46:519–525.PubMedGoogle Scholar
  74. 74.
    Minden, P., Houghton, R. A., Spear, J. R., and Shinnick, T. M., 1986, A chemically synthesized peptide which elicits humoral and cellular immune response to mycobac-terial antigens, Infect. Immun. 53:560–564.PubMedGoogle Scholar
  75. 75.
    Affronti, L. F., Wright, G. L., Jr., and Reich, M., 1972, Characterization and comparison of mycobacterial antigens by discontinuous pore gradient gel electrophoresis, Infect. Immun. 5:474–481.PubMedGoogle Scholar
  76. 76.
    Wright, G. L., Jr., Affronti, L. F., and Reich, M., 1972, Characterization and comparison of mycobacterial antigens by two-dimensional polyacrylamide gel electrophoresis, Infect. Immun. 5:482–490.PubMedGoogle Scholar
  77. 77.
    Roszman, T. L., Mallmann, V. H., Mallmann, W. L., and O’Reilly, D., 1968, Immu-nologic responses evoked by mycobacterial components. Disc electrophoretic separation of specific mycobacterial antigens, Am. Rev. Respir. Dis. 97:103–112.Google Scholar
  78. 78.
    Minden, P., and Farr, R. S., 1969, Binding between components of the tubercle bacillus and humoral antibodies, J. Exp. Med. 130:931–954.PubMedCrossRefGoogle Scholar
  79. 79.
    Affronti, L. F., Grow, L., Ouchterlony, O., Lind., A., and Norlin, M., 1971, Fractiona-tion of the international standard tuberculin, PPD-S, Am. Rev. Respir. Dis. 103:893 (abst.)Google Scholar
  80. 80.
    Affronti, L. F., Lind, A., Ouchterlony, O., Ridell, M., and Wisingerova, E., 1986, An evaluation of the polyacrylamide gel electrophoresis fractionation method for the production of Mycobacterium tuberculosis skin test preparation. I. Production, phys-icochemical characterization, and serological analysis, J. Biol. Std. 14:177–185.CrossRefGoogle Scholar
  81. 81.
    Affronti, L. F., Bentzon, M. W., Lind, A. ,Magnusson, M., Ouchterlony, O., Ridell, M., and Wisingerova, E., 1986, An evaluation of the polyacrylamide gel electrophoresis fractionation method for the production of Mycobacterium tuberculosis skin test preparations. II. Comparative skin testing on guinea pigs, J. Biol. Std. 14:187– 200.CrossRefGoogle Scholar
  82. 82.
    Oakley, C. L., and Fulthorpe, A. J., 1953, Antigenic analysis by diffusion, J. Pathol. 65:49–60.CrossRefGoogle Scholar
  83. 83.
    Parlett, R. C., Youmans, G. P., Rehr, C., and Lester, W., 1958, The detection of antibodies in the serum of tuberculous patients by an agar double diffusion pre-cipitatin technique. Am. Rev. Tuberc. 77:462–472.PubMedGoogle Scholar
  84. 84.
    Parlett, R. C., and Youmans, G. P., 1959, An evaluation of the specificity and sensitivity of a gel double diffusion test for tuberculosis, Am. Rev. Respir. Dis.. 80: 153–166.PubMedGoogle Scholar
  85. 85.
    Lind, A., 1961, A comparison between the plate and tube technique for the demonstration of precipitins in sera from tuberculous patients, Int. Arch. Allergy. Appl. Immunol. 18:305–317.PubMedCrossRefGoogle Scholar
  86. 86.
    Grange, J. M., and Kardjito, T., 1982, Serological tests for tuberculosis: Can the problem of how specificity be overcome? Indian J. Chest. Dis. 24:108–117.Google Scholar
  87. 87.
    Thurston, J. R., 1964, The present status of serologic test for the diagnosis of tuberculosis, Proc. U.S. Arum. Health Assoc. 68:314–319.Google Scholar
  88. 88.
    Affronti, L. F., and Seibert, F. B., 1965, Some early investigations of Mycobacterium tuberculosL, Am. Rev. Respir. Dis. 92:1–8.PubMedGoogle Scholar
  89. 89.
    Toussaint, A. J., Fife, E. H., Parlett, R. C., Affronti, L. F., Wright, G. L., Jr., Reich, M., and Morse, W. C., 1969, A soluble antigen fluorescent antibody test for the serodiagnosis of Mycobacterium tuberculosis infections, Am. J. Clin. Pathol. 52:708–713.PubMedGoogle Scholar
  90. 90.
    Affronti, L. F., Fife, E. H., and Grow, L., 1973, Serodiagnostic test for tuberculosis, Am. Rev. Respir. Dis. 108:822–825.Google Scholar
  91. 91.
    Kiran, U., and Shriniwas, 1985, Soluble antigen fluorescent antibody test in serodiagnosis of pulmonary tuberculosis, Eur. J. Respir. Dis.. 67:204–208.PubMedGoogle Scholar
  92. 92.
    Ellner, J. J., and Daniel, T. M., 1979, Immunosuppression by mycobacterial ara-binomannan, Clin. Exp. Immunol. 35:250–257.PubMedGoogle Scholar
  93. 93.
    Minden, P., McClatchy, J. K., and Farr, R. S., 1972, Shared antigens between hetero-logous bacterial species, Infect. Immun. 6:574–582.PubMedGoogle Scholar
  94. 94.
    Straus, E., Wu, N., Quraishi, M. A. H., and Levine, S., 1981, Clinical applications of the radioimmunoassay of secretory tuberculin protein, Proc. Natl. Acad. Sci. USA 78:3214–3217.PubMedCrossRefGoogle Scholar
  95. 95.
    Engvall, E., and Perlmann, P., 1972, Enzyme-linked immunosorbent assay (ELISA). III. Quantitation of specific antibodies by enzyme labeled anti-immunoglobulin in antigen coated tubes, J. Immunol. 109:129–135.PubMedGoogle Scholar
  96. 96.
    Nassau, E., Parson, E. R., and Johnson, G. D., 1976, The detection of antibodies to Mycobacterium tuberculosis by enzyme-linked immunosorbent assay (ELISA), Tubercle 57:67–70.PubMedCrossRefGoogle Scholar
  97. 97.
    Tandon, A., Saxena, R. P., and Saxena, K. C., 1980, Diagnostic potentialities of enzyme-linked immunosorbent assay in tuberculosis using purified tuberculin antigen, Tubercle 61:87–89.PubMedCrossRefGoogle Scholar
  98. 98.
    Grange, J. M., Gibson, J., Nassau, E., and Kardjito, T., 1980, Enzyme-linked immu nosorbent assay (ELISA): A study of antibodies of Mycobacterium tuberculosis in the IgG, IgA, and IgM classes in tuberculosis, sarcoidosis, and Crohn’s disease, Tubercle 61:145–152.PubMedCrossRefGoogle Scholar
  99. 99.
    Kardjito, T., Handayo, I., and Grange, J. M., 1982, Diagnosis of active tuberculosis by immunological methods. I. The effect of tuberculin reactivity and previous BCG vaccination on the antibody levels determined by ELISA, Tubercle 63:269–274.PubMedCrossRefGoogle Scholar
  100. 100.
    Reggiardo, I., Vasquez, E., and Schnoper, L., 1980, ELISA tests for antibodies against mycobacteria glycolipids, J. Immunol. Methods 34:55–60.PubMedCrossRefGoogle Scholar
  101. 101.
    Daniel, T. M., Oxtoby, M. J., Pinto, E. M., and Moreno, E. S., 1981, The immune spectrum in patients with pulmonary tuberculosis, Am. Rev. Respir. Dis. 123:556–559.PubMedGoogle Scholar
  102. 102.
    Viljanen, M. K., Eskola, J., and Tala, E., 1982, Enzyme-linked immunosorbent assay (ELISA) for antibodies to purified protein derivative of tuberculin PPD, Eur. J. Respir. Dis. 63:257–262.PubMedGoogle Scholar
  103. 103.
    Kalish, S. B., Radin, R. C., Levitz, D., Zeiss, C. R., and Phair, J. P., 1983, The enzyme linked immunosorbent assay method for IgG antibody to purified protein derivative in cerebrospinal fluid of patients with tuberculous meningitis, Ann. Intern. Med. 99:630–633.PubMedGoogle Scholar
  104. 104.
    Affronti, L. F., 1985, Useful immunologic tests for tuberculosis, Clin. Immunol. Newsl. 6:55–57.CrossRefGoogle Scholar
  105. 105.
    Affronti, L. F., Taylor, R., and Hassan, E. M., 1986, Comparison between tuberculoprotein A and C in the serodiagnosis of tuberculosis by enzyme-linked immunosorbent assay, Int. Arch. Allergy Appl. Immunol. 80:81–84.PubMedCrossRefGoogle Scholar
  106. 106.
    Vandiviere, H. M., Vandiviere, H. R., and Seibert, F. B., 1961, Comparison between purified tuberculoproteins and the international standard tuberculin, J. Infect. Dis. 108:45–52.PubMedCrossRefGoogle Scholar
  107. 107.
    Sada, E., Ruiz-Palacios, G. M., L’opez-Vidal Y., and Ponce de Le’on, S., 1983, Detection of mycobacterial antigens in cerebrospinal fluid of patients with tuberculous meningitis by enzyme-linked immunosorbent assay, Lancet 2:651–652.PubMedCrossRefGoogle Scholar
  108. 108.
    Yanez, M. A., Coppola, M. P., Russo, D. A., Delaha, E., Chaparas, S. D., and Yeager, H., Jr., 1986, Determination of mycobacterial antigens in sputum by enzyme immunoassay, J. Clin. Microbiol. 23:822–825.PubMedGoogle Scholar
  109. 109.
    Coates, S. R., Hansen, D., Schecter, G., Slutkin, G., Hopewell, P., Affronti, L. F., and Echenberg, D. F., 1986, Identification of Mycobacterium tuberculosis antigens in Seibert fractions by immunoblotting, J. Clin. Microbiol. 24: 126–130.PubMedGoogle Scholar
  110. 110.
    Clark-Curtiss, J. E., Jacobs, W. R., Docherty, M. A., Ritchis, L. R., and Curtiss, R. III, 1985, Molecular analysis of DNA and construction of genomic libraries of Mycobacterium leprae, J. Bacteriol. 161:1093–1102.PubMedGoogle Scholar
  111. 111.
    Young, R. A., Bloom, B. R., Grosskinsky, C. M., Ivanyi, J., Thomas, D., and Davis, R., 1985, Dissection of Mycobacterium tuberculosis antigens using recombinant DNA, Proc. Natl. Acad. Sci. USA 82:2583–2587.PubMedCrossRefGoogle Scholar
  112. 112.
    Thole, J. E. R., Dauwerse, H. G., Das, R. K., Groothuis, D. G., Schouls, L. M., and van Embden, J. D. A., 1985, Cloning of Mycobacterium bovis BCG DNA and expression of antigens in Escherichia coli, Infect. Immun. 50:800–806.Google Scholar
  113. 113.
    Coates, A. R. M., Allen, B. W., Hewitt, J., Ivanyi, J., and Mitchison, D. A., 1981, Antigenic diversity of Mycobacterium tuberculosis and Mycobacterium bovis detected by means of monoclonal antibodies, Lancet 2: 167–169.PubMedCrossRefGoogle Scholar
  114. 114.
    Kolk, A. H. J., Ho, M. L., Klatser, P. R., Eggelte, T. A., Kuijper, S., dejonge, S., and van Leeuwen, J., 1984, Production and characterization of monoclonal antibodies to Mycobacterium tuberculosis, M. bovis (BCG) and M. leprae, Clin. Exp. Immunol. 58:511 -521.PubMedGoogle Scholar
  115. 115.
    Andersen, A. B., Yuan, Z. ,Haslov, K., Vergmann, B., and Bennedsen, J. ,1986, Interspecies reactivity of five monoclonal antibodies to Mycobacterium tuberculosis as examined by immunoblotting and enzyme-linked immunosorbent assay, J. Clin. Mi crobiol. 23:446–451.Google Scholar
  116. 116.
    Chandramuki, A. ,Allen, P. R., Keen, M. ,and Ivanyi, J., 1985, Detection of mycobacterial antigen and antibodies in the cerebrospinal fluid of patients with tuberculous meningitis, J. Med. Microbiol. 20:239–247.PubMedCrossRefGoogle Scholar
  117. 117.
    Praputpittaya, K., and Ivanyi, J., 1985, Detection of an antigen (MY4) common toM. tuberculosis and M. leprae by “tandem” immunoassay, J. Immunol. Methods 79:149–157.PubMedCrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1988

Authors and Affiliations

  • L. F. Affronti
    • 1
  1. 1.Department of MicrobiologyThe George Washington University Medical CenterUSA

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