Hippocampal Stimulation of Cholinergic Expression in Primary Cultures of Basal Forebrain Neurons

  • Bradley C. Wise
  • Michel B. Emerit
Part of the Wenner-Gren Center International Symposium Series book series (WGCISS)


A major goal of development neurobiology is to define the cellular and molecular mechanisms which regulate the survival, growth and synaptogenesis of central nervous system neurons. A role for specific neurotrophic factors in CNS development has been postulated based on described processes occurring in the peripheral nervous system such as naturally occurring neuronal death (Cowan et al., 1984). Such a process might serve to match the size of the afferent neuronal population with that of the target area of innervation. A factor determining neuronal death might be neuronal competition for a limited quantity of trophic factors present in the target region. The identification, characterization and our present understanding of the physiology of the nerve growth factor (NGF) emphasizes the importance of target-drived molecules on the survival, growth and differentiation of an afferent neuronal population (Thoenen and Barde, 1980; Yanker and Shooter, 1982). In mature neurons the maintenance of a critical number of terminal boutons may also require trophic support, albeit at a much lower level than is needed during development. Neurodegenerative diseases, such as senile and presenile dementias, may be caused in part by abnormalities in the supply of neurotrophic factors specific for certain neurons (Appel, 1981).


Nerve Growth Factor Cholinergic Neuron Basal Forebrain Hippocampal Cell Choline Uptake 
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  1. Appel, S.H. (1981). A unifying hypothesis for the cause of amyotrophic lateral sclerosis, parkinsonism, and Alzheimer disease. Ann. Neurol., 10, 499–505.CrossRefGoogle Scholar
  2. Bottenstein, J.E., and Sato, G.H. (1979). Growth of a rat neuroblastoma cell line in serum-free supplemented medium. Proc. Natl. Acad. Sci. USA, 76, 514–517.CrossRefGoogle Scholar
  3. Campenot, R.B. (1982). Development of sympathetic neurons in compartmentalized cultures. II. Local control of neurite survival by nerve growth factor. Dev. Biol., 93, 13–21.CrossRefGoogle Scholar
  4. Cowan, W.M., Fawcett, J.W., O’Leary, D.D.M., and Stanfield, B.B. (1984). Regressive events in neurogenesis. Science, 225, 1258–1265.CrossRefGoogle Scholar
  5. Di Porzio, U., Daguet, M.C., Glowinski, J., and Prochiantz, A. (1980). Effect of striatal cells on in vitro maturation of mesencephalic dopaminergic neurones grown in serum-free conditions. Nature, 288, 370–373.CrossRefGoogle Scholar
  6. Di Porzio, U., and Estenoz, M. (1984). Positive control of target cerebellar cells on norepinephrine uptake in embryonic brainstem cultures in serum-free medium. Dev. Brain Res., 16, 147–157.CrossRefGoogle Scholar
  7. Eva, C., Hadjiconstantinou, M., Neff, N.H., and Meek, J.L. (1984). Acetylcholine measurement by high-performance liquid chromatography using an enzyme-loaded postcolumn reactor. Anal. Biochem., 143, 320–324.CrossRefGoogle Scholar
  8. Gahwiler, B.H., and Brown, D.A. (1985). Functional innervation of cultured hippocampal neurones by cholinergic afferents from co-cultured septal explants. Nature, 313, 577–579.CrossRefGoogle Scholar
  9. Gahwiler, B.H., and Hefti, F. (1984). Guidance of acetylcholinesterase-containing fibres by target tissue in co-cultured brain slices. Neurosci., 13, 681–689.CrossRefGoogle Scholar
  10. Hefti, F., Hartikka, J., Eckenstein, F., Gnahn, H., Heumann, R., and Schwab, M. (1985). Nerve growth factor increases choline acetyltransferase but not survival or fiber outgrowth of cultured fetal septal cholinergic neurons. Neurosci., 14, 55–68.CrossRefGoogle Scholar
  11. Hemmendinger, L.M., Garber, B.B., Hoffman, P.C., and Heller, A. (1981). Target neuron-specific process formation by embryonic mesencephalic dopamine neurons in vitro. Proc. Natl. Acad. Sci. USA, 78, 1264–1268.CrossRefGoogle Scholar
  12. Johnson, D.A., and Pilar, G. (1980). The release of acetylcholine from post-ganglionic cell bodies in response to depolarization. J. Physiol., 299, 605–619.Google Scholar
  13. Letourneau, P.C. (1983). Axonal growth and guidance. Trends in Neurosci., 6, 451–455.CrossRefGoogle Scholar
  14. Rimvall, K., Keller, F., and Waser, P.G. (1985). Development of cholinergic projections in organotypic cultures of rat septum, hippocampus and cerebellum. Dev. Brain Res., 19, 267–278.CrossRefGoogle Scholar
  15. Thoenen, H., and Barde, Y.A. (1980). Physiology of nerve growth factor. Physiol. Rev., 60, 1284–1335.Google Scholar
  16. Tuttle, J.B., Vaca, K., and Pilar, G. (1983). Target influences on [3H]Ach synthesis and release by ciliary ganglion neurons in vitro. Dev. Biol., 97, 255–263.CrossRefGoogle Scholar
  17. Yankner, B.A., and Shooter, E.M. (1982). The biology and mechanism of action of nerve growth factor. Ann. Rev. Biochem., 51, 845–868.CrossRefGoogle Scholar

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© The Wenner-Gren Center 1987

Authors and Affiliations

  • Bradley C. Wise
  • Michel B. Emerit

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