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Receptor Modification and Receptor Disposition in Membranes

  • Deirdre Cooney
  • Andreas Holzhöfer
  • Fritz Boege
  • Christian Dees
  • Rolf Jürss
  • Alan K. Keenan
  • Mirko Hekman
  • Ernst J. M. Heimreich
Part of the Wenner-Gren Center International Symposium Series book series (WGCISS)

Abstract

This report deals with the functional significance of covalent modifications of the ß1-adrenoceptor from turkey erythrocytes. Two types of modification are being considered: phosphorylation and glycosylation. The effects of these modifications were tested using a reconstitution assay with pure receptor and GTP-binding proteins incorporated into lipid vesicles.

Keywords

Adenylate Cyclase Covalent Modification GTPase Activity Lipid Vesicle Culture Liver Cell 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Benovic, J.L., Strasser, R.H., Caron, M.G. and Lefkowitz, R.J. (1986). ß-Adrenergic receptor kinase: Identification of a novel protein kinase that phosphorylates the agonist-occupied form of the receptor. Proc. Natl. Acad. Sci. USA 83, 2797–2801.CrossRefGoogle Scholar
  2. Boege, F., Jürss, R., Cooney, D., Hekman, M., Keenan, A.K. and Helm-reich, E.J.M. (1986). Functional and structural characterization of the two ß1-adrenoceptor forms in turkey erythrocytes with Mr weights of 50 kDa and 40 kDa. Biochemistry, in press.Google Scholar
  3. Burgermeister, W., Hekman, M. and Helmreich, E.J.M. (1982). Photo-affinity labeling of the ß-adrenergic receptor with azide derivatives of iodocyanopindolol. J. Biol. Chem. 257, 5306–5311.Google Scholar
  4. Cassel, D. and Selinger, Z. (1976). Catecholamine-stimulated GTPase activity in turkey erythrocyte membranes. Biochim. Biophys. Acta 452, 538–551.Google Scholar
  5. Feder, D., Im, M.J., Klein, H.W., Hekman, M., Holzhöfer, A., Dees, C., Levitzki, A., Helmreich, E.J.M. and Pfeuffer, T. (1986). Reconstitution of 81-adrenoceptor-dependent adenylate cyclase from purified components. EMBO J. 5, 1509–1514.Google Scholar
  6. Hekman, M., Feder, D., Keenan, A.K., Gal, A., Klein, H.W., Pfeuffer, T., Levitzki, A. and Helmreich, E.J.M. (1984). Reconstitution of ß-adrenergic receptor with components of adenylate cyclase. EMBO J. 3, 3339–3345.Google Scholar
  7. Helmreich, E.J.M. and Elson, E.L. (1984). Mobility of proteins and lipids in membranes. Adv. Cyclic Nucleotide and Protein Phosphorylation Res. 18, 1–62.Google Scholar
  8. Henis, Y.I., Hekman, M., Elson, E.L. and Helmreich, E.J.M. (1982). Lateral motion of ß-receptors in membranes of cultured liver cells. Proc. Natl. Acad. Sci. USA 79, 2907–2911.CrossRefGoogle Scholar
  9. Iyengar, R. and Birnbaumer, L. (1982). Hormone receptor modulates the regulatory component of adenylyl cyclase by reducing its requirement for Mg2+ and enhancing its extent of activation by guanine nucleotides. Proc. Natl. Acad. Sci. USA 79, 5179–5183.CrossRefGoogle Scholar
  10. Jürss, R., Hekman, M. and Helmreich, E.J.M. (1985). Proteolysis-associated deglycosylation of ß1-adrenergic receptor in turkey erythrocytes and membranes. Biochemistry 24, 3349–3354.CrossRefGoogle Scholar
  11. Nambi, P., Peters, J.R., Sibley, D.R. and Lefkowitz, R.J. (1985). Desensitization of the turkey erythrocyte 8-adrenergic receptor in a cell-free system. J. Biol. Chem. 260, 2165–2171.Google Scholar
  12. Simpson, I.A. and Pfeuffer, T. (1980). Functional desensitization of 8-adrenergic receptors of avian erythrocytes by catecholamines and adenosine 3’,5’-phosphate. Eur. J. Biochem. 111, 111–116.CrossRefGoogle Scholar
  13. Stadel, J.M., Nambi, P., Shorr, R.G.L., Sawyer, D.F., Caron, M.G. and Lefkowitz, R.J. (1983). Catecholamine-induced desensitization of turkey erythrocyte adenylate cyclase is associated with phosphorylation of the ß-adrenergic receptor. Proc. Natl. Acad. Sci. USA 80, 3173–3177.CrossRefGoogle Scholar
  14. Strulovici, B., Cerione, R.A., Kilpatrick, B.F., Caron, M.G. and Lefkowitz, R.J. (1984). Direct demonstration of impaired functionality of a purified desensitized 8-adrenergic receptor in a reconstituted system. Science 225, 837–840.CrossRefGoogle Scholar
  15. Wright, D.E. and Rodbell, M. (1980) Preparation of 2-thioltryptophan-glucagon and (tryptophan-S-glucagon)2. J. Biol. Chem. 255, 10884–10887.Google Scholar

Copyright information

© The Wenner-Gren Center 1987

Authors and Affiliations

  • Deirdre Cooney
  • Andreas Holzhöfer
  • Fritz Boege
  • Christian Dees
  • Rolf Jürss
  • Alan K. Keenan
  • Mirko Hekman
  • Ernst J. M. Heimreich

There are no affiliations available

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