Cellular Models of Epithelial Ion Transport

  • Stanley G. Schultz


The first cellular model of epithelial ion transport was proposed in 1958 by Koefoed-Johnsen and Ussing(1) (KJU) to account for the relation between active Na+ transport and the electrical potential difference across isolated frog skin. The essential feature of this now-classic model (Fig. 1) was that the epithelial cell could be viewed as two membranes arranged in series separated by a homogeneous cytoplasmic compartment with net transcellular or vectorial transport resulting from the asymmetric properties of the two limiting barriers. In the case of frog skin, the outer or apical membrane was presumed to be permselective to Na+ and scarcely if at all permeable to K+. The inner or basolateral membrane, on the other hand, was presumed to be permselective to K+, scarcely if at all permeable to Na+, and to possess an active pump mechanism that extrudes Na+ from the cell in exchange for K+. This asymmetric arrangement of pump and leaks could simultaneously account for active transcellular Na+ transport as well as the maintenance of the low intracellular Na+ concentration and the high intracellular K+ concentration characteristic of virtually all cells of higher animals. Because of its simplicity—involving only transport mechanisms that had already been reasonably well established by studies on nonepithelial cells—this model was rapidly accepted and elevated to the status of a paradigm.


Apical Membrane Basolateral Membrane Frog Skin Chloride Transport Electrical Potential Difference 
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Copyright information

© Plenum Publishing Corporation 1987

Authors and Affiliations

  • Stanley G. Schultz
    • 1
  1. 1.Department of Physiology and Cell BiologyUniversity of Texas Medical SchoolHoustonUSA

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