Ovarian Follicular Fluid Peptide (OFFP): Effects on Follicular Maturation, Ovulation and Luteinization in Mice and Marmosets
Intraovarian concentrations of steroids and non-steroidal factors have recently been reported by Demoalin and Franchimont (1985) to play an important role in deciding the fate of the follicle. We have attempted to partially purify and characterize a peptide from sheep follicular fluid (OFFP). The pooled follicular fluid was chromatographed on Sephadex G-100 and later on Sephadex G-25 column (Kadam et al., 1984). The bioactive fraction (GF2) inhibited maturation of large follicles and ovulation in cycling mice. It also decreased plasma progesterone (P) levels but did not affect FSH levels significantly in these mice (Nandedkar et al., 1986). It reduced 33 hydroxysteroid dehydrogenase (33 HSD) activity in mouse granulosa cells. Further, the purified Fraction 1 on high performance liquid chromatography also inhibited 3 3 HSD activity in mouse granulosa cells. The trypsin digestion and heat inactivation of the peptide indicated the heat labile nature of OFFP (Kadam et al., 1984).
KeywordsGranulosa Cell Follicular Fluid Heat Inactivation Large Follicle Hydroxysteroid Dehydrogenase
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