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Ovarian Follicular Fluid Peptide (OFFP): Effects on Follicular Maturation, Ovulation and Luteinization in Mice and Marmosets

  • T. D. Nandedkar
  • A. L. Kadam
  • S. D. Kholkute
  • N. J. Joshi
  • S. B. Moodbidri
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 219)

Abstract

Intraovarian concentrations of steroids and non-steroidal factors have recently been reported by Demoalin and Franchimont (1985) to play an important role in deciding the fate of the follicle. We have attempted to partially purify and characterize a peptide from sheep follicular fluid (OFFP). The pooled follicular fluid was chromatographed on Sephadex G-100 and later on Sephadex G-25 column (Kadam et al., 1984). The bioactive fraction (GF2) inhibited maturation of large follicles and ovulation in cycling mice. It also decreased plasma progesterone (P) levels but did not affect FSH levels significantly in these mice (Nandedkar et al., 1986). It reduced 33 hydroxysteroid dehydrogenase (33 HSD) activity in mouse granulosa cells. Further, the purified Fraction 1 on high performance liquid chromatography also inhibited 3 3 HSD activity in mouse granulosa cells. The trypsin digestion and heat inactivation of the peptide indicated the heat labile nature of OFFP (Kadam et al., 1984).

Keywords

Granulosa Cell Follicular Fluid Heat Inactivation Large Follicle Hydroxysteroid Dehydrogenase 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Darga NC, Reichert LE Jr, 1978. Some properties of the interaction of follicular stimulating hormone with bovine granulosa cells and its inhibition by follicular fluid. Bio Reprod 19:235–240CrossRefGoogle Scholar
  2. Demoalin A, Franchimont P, 1985. Ovarian hormones and cybermins. In: Runnebaum B, Rabe T, Kiesel L (eds.), Future Aspects in Contraception. Boston: MTP Press, pp 281–288CrossRefGoogle Scholar
  3. Kadam AL, Moodbidri SB, Nandedkar TD, 1984. Inhibition of 33 hydroxysteroid dehydrogenase (3β HSD) activity in the granulosa cells of mouse ovarian follicles by follicular fluid peptide. Steroids 44:497–509PubMedCrossRefGoogle Scholar
  4. Ledwitz-Rigby F, Rigby BW, 1981. Ovarian inhibitors and stimulators of granulosa cells maturation and luteinization. In: Franchimont P, Channing CP (eds.), Intragonadal Regulation of Reproduction. New York: Academic Press, pp 97–131Google Scholar
  5. Nandedkar TD, Kadam AL, Moodbidri SB, 1986. Control of follicular maturation in mouse by a non-steroidal regulator from sheep follicular fluid. Int J Fertil (In Press)Google Scholar
  6. Tonetta SA, diZerega GS, 1986. Paracrine regulation of follicular maturation in primates. Clinics in Endocrinol and Metab 15:135–150CrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1987

Authors and Affiliations

  • T. D. Nandedkar
    • 1
  • A. L. Kadam
    • 1
  • S. D. Kholkute
    • 1
  • N. J. Joshi
    • 1
  • S. B. Moodbidri
    • 1
  1. 1.Institute for Research in Reproduction (ICMR)BombayIndia

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