Paracrine Control of 3β-Hydroxysteroid Dehydrogenase, 17,20-Lyase and Aromatase Enzyme Systems in Porcine Thecal Cells

  • Sharon A. Tonetta
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 219)


Thecal cells are important intraovarian sources of androgens, providing substrate necessary for the synthesis of estradiol by granulosa cells (Armstrong and Dorrington, 1977), However, in addition to androgens, progesterone and estradiol are secreted by thecal cells of sows (Stoklosowa et al., 1982), cows (McNatty et al., 1984), monkeys (Vernon et al., 1983) and humans (McNatty et al., 1979). Although thecal steroidogenesis is regulated partly by circulating levels of luteinizing hormone (Baird and McNeilly, 1981; Merz et al., 1981; Carlson et al., 1984), the intrafollicular control of thecal steroidogenesis is unclear. As local steroidal and nonsteroidal factors can modify granulosa cell steroidogenesis in vitro (Hsueh et al., 1984; Tonetta and diZerega, 1986), the effects of physiological concentrations of steroids and gonadotropin on cultured porcine thecal cells were determined.


Luteinizing Hormone Granulosa Cell Aromatase Activity Lyase Activity Thecal Cell 
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  1. Armstrong DT, Dorrington JH, 1977. Estrogen biosynthesis in the ovaries and testis. In: Thomas JA, Singhai RL (eds.), Regulatory Mechanisms Affecting Gonadal Hormonal Action. Baltimore: University Park Press, 3:217Google Scholar
  2. Baird DT, McNeilly AS, 1981. Gonadotropic control of follicular development and function during the estrous cycle of the ewe. J Reprod Fertil 30:119–133Google Scholar
  3. Battin DA, diZerega GS, 1985. Effect of human menopausal gonadotropin and follicle regulatory protein(s) on 3 β-hydroxysteroid dehydrogenase in human granulosa cells. J Clin Endocrinol Metab 60:1116–19PubMedCrossRefGoogle Scholar
  4. Carlson RS, Findlay JK, Clarke IJ, Burger HG, 1984. Estradiol, ketosterone, and androstenedione in ovine follicular fluid during growth and atresia of ovarian follicles. Biol Reprod 24:105–13CrossRefGoogle Scholar
  5. Dorrington JH, Moon YS, Armstrong DT, 1975. Estradiol-17ß biosynthesis in cultured granulosa cells from hypophysectomized immature rats: stimulation by follicle-stimulating hormones. Endocrinology 97:1328–31PubMedCrossRefGoogle Scholar
  6. Erickson GF, Ryan KJ, 1976. Stimulation of ketosterone production in isolated rabbit thecal tissue by LH/FSH, dibutyrl cyclic AMP, PGFα. and PGE2. Endocrinology 99:452–58PubMedCrossRefGoogle Scholar
  7. Fortune JE, Vincent SE, 1983. Progesterone inhibits the induction of aromatase activity in rat granulosa cells in vitro. Biol Reprod 28:1078–89PubMedCrossRefGoogle Scholar
  8. Gore-Langton RE, Dorrington JH, 1981. FSH induction of aromatase in cultured rat granulosa cells measured by a radiometric assay. Mol Cell Endocrinol 22:135–51PubMedCrossRefGoogle Scholar
  9. Haney AF, Schomberg DW, 1978. Steroidal modulation of progesterone secretion by granulosa cells from large porcine follicles: a role for androgens and estrogens in controlling steroidogenesis. Biol Reprod 19:252–48CrossRefGoogle Scholar
  10. Hsueh AJW, Adashi EY, Jones PBC, Welsh Jr TH, 1984. Hormonal regulation of the differentiation of cultured ovarian granulosa cells. Endocrine Rev 5:76–127CrossRefGoogle Scholar
  11. Johnson DC, Griswold T, 1985. Ovarian C17–20-lyase: changes in intact and hypophysectomized immature rats treated with pregnant mare’s serum gonadotropin. J Steroid Biochem 20:733–39CrossRefGoogle Scholar
  12. Jones PBC, Hsueh AJW, 1982. Regulation of ovarian 3β -hydroxysteroid dehydrogenase activity by gonadotropin-releasing hormone and follicle-stimulating hormone in cultured rat granulosa cells. Endocrinology 110:1663–71PubMedCrossRefGoogle Scholar
  13. Lieberman ME, Barnea A, Bauminger S, Tsafriri A, Collins WP, Lindner HR, 1975. LH effect on the pattern of steroidogenesis in cultured Graafian follicles of the rabbit: dependence on macromolecular synthesis. Endocrinology 96:1533–42PubMedCrossRefGoogle Scholar
  14. Lucky AW, Schreiber JR, Hillier SG, Schulman JD, Ross GT, 1977. Progesterone production by cultured preantral rat granulosa cells: stimulation by androgens. Endocrinology 100:128–33PubMedCrossRefGoogle Scholar
  15. Makris A, Ryan KJ, 1975. Progesterone, androstenedione, testosterone, estrone and estradiol synthesis in hamster ovarian follicle cells. Endocrinology 96:694–701PubMedCrossRefGoogle Scholar
  16. McNatty KP, Makris A, Osathanondh R, Ryan KJ, 1980. Effects of luteinizing hormone on steroidogenesis by thecal tissue from human ovarian follicles in vitro. Steroids 36:53–63PubMedCrossRefGoogle Scholar
  17. McNatty KP, Heath DA, Lun S, Fannin JM, McDiarmid JM, Henderson KM, 1984. Steroidogenesis by bovine theca internal in an in vitro perfusion system. Biol Reprod 30:159–70PubMedCrossRefGoogle Scholar
  18. McNatty KP, Smith DM, Makris A, Osathanondh R, Ryan KJ, 1979. The microenvironment of the human antral follicle: interrelationships among the steroid. Fertil Steril 32:433–438PubMedGoogle Scholar
  19. Merz EA, Hauser ER, England BG, 1981. Ovarian function in the cycling cow: relationship between gonadotropin binding to theca and granulosa and steroidogenesis in individual follicles. J Anim Sci 52:1457–68PubMedGoogle Scholar
  20. Mills TM, 1975. Effect of luteinizing hormone and cyclic adenosine 3’, 5’- monophosphate on steroidogenesis in the ovarian follicle of the rabbit. Endocrinology 96:440–54PubMedCrossRefGoogle Scholar
  21. Moon YS, Dorrington JH, Armstrong DT, 1975. Stimulating action of follicle stimulating hormone on estradiol-17β secretion by hypophysectomized rat ovaries in organ culture. Endocrinology 97:244–47PubMedCrossRefGoogle Scholar
  22. Quinn PG, Payne AH, 1985. Oxygen-mediated damage of microsomal cytochrome P-450 enzymes in cultured Leydig cells. J Biol Chem 259:4130–35Google Scholar
  23. Samuels LM, Helreich M, Lasater M, Reich H, 1951. An enzyme in endocrine tissues which oxidizes Δ5–3-hydroxy steroid to a β-unsaturated ketones. Science 113:490–91PubMedCrossRefGoogle Scholar
  24. Schomberg DW, Stouffer RL, Tyrey L, 1976. Modulation of progestin secretion in ovarian cells by 17β -hydroxy-5α -androstan-3-one (dihydrostenedione): a direct demonstration in monolayer culture. Biochem Biophys Res Commun 68:77–8PubMedCrossRefGoogle Scholar
  25. Schreiber JR, Nakamura K, Erickson GF, 1980. Progestins inhibit FSH-stimulated steroidogenesis in cultured rat granulosa cells. Mol Cell Endocrinol 19:165–73PubMedCrossRefGoogle Scholar
  26. Schreiber JR, Nakamura K, Erickson GF, 1981. Progestins inhibit FSH-stimulated granulosa cell estrogen production at a postcyclic AMP site. Mol Cell Endocrinol 21:161–70PubMedCrossRefGoogle Scholar
  27. Stoklosowa S, Gregoraszcsuk E, Channing CP, 1982. Estrogen and progesterone secretion by isolated cultured porcine thecal and granulosa cells. Biol Reprod 26:943–52PubMedCrossRefGoogle Scholar
  28. Tonetta SA, De Vinna RS, diZerega GS, 1986. Modulation of porcine thecal cell aromatase activity by human chorionic gonadotropin, progesterone, estradiol-17β and dihydrotestosterone. Biol Reprod 35:785–91PubMedCrossRefGoogle Scholar
  29. Tonetta SA, diZerega GS, 1986. Paracrine regulation of follicular maturation in primates. Clinics in Endocrinology and Metabolism 15:135–36PubMedCrossRefGoogle Scholar
  30. Tsang BK, Ainsworth L, Downey BR, Marcus GJ, 1985. Differential production of steroids by dispersed granulosa and theca interna cells from developing preovulatory follicles of pigs. J Reprod Fertil 74:459–71PubMedCrossRefGoogle Scholar
  31. Tsang BK, Moon YS, Simpson CW, Armstrong DT, 1979. Androgen biosynthesis in human ovarian follicles: cellular source, gonadotropic control, and adenosine 3’,5’-monophosphate mediation. J Clin Endocrinol Metab 48:153–58PubMedCrossRefGoogle Scholar
  32. Tseng L, 1984. Effect of estradiol and progesterone on human endometrial aromatase activity in primary cell culture. Endocrinology 115:833–35PubMedCrossRefGoogle Scholar
  33. Vernon MW, Dierschke DJ, Sholl SA, Wolf RC, 1983. Ovarian aromatase activity in granulosa and theca cells of rhesus monkeys. Biol Reprod 28:342–49PubMedCrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1987

Authors and Affiliations

  • Sharon A. Tonetta
    • 1
  1. 1.Livingston Biological Research LaboratoryUniversity of Southern California Medical SchoolLos AngelesUSA

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