Several GnRH Stimulation Frequencies Differentially Release FSH and LH from Isolated, Perfused Rat Anterior Pituitary Cells

  • R. T. Savoy-Moore
  • K. H. Swartz
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 219)


Gonadotropin-releasing hormone (GnRH) releases both follicle-stimulating hormone (FSH) and luteinizing hormone (LH) from the anterior pituitary, but not always at the same time. Both hormones surge on the day of proestrus in the rat, but only the FSH surge persists through estrous morning (Butcher et al., 1975; Smith et al., 1975). One way that a single releasing hormone could differentially affect two gonadotropins is by variation of the GnRH pulse pattern. Both frequency and amplitude have been altered to release more FSH than LH (Wildt et al., 1981; Wise et al., 1978).


Luteinizing Hormone Estrous Cycle Releasable Luteinizing Hormone Anterior Pituitary Cell Luteinizing Hormone Release 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Butcher RL, Collins WE, Fugo NW, 1975. Altered secretion of gonadotropins and steroids resulting from delayed ovulation in the rat. Endocrinology 96:576–586PubMedCrossRefGoogle Scholar
  2. Levine JE, Ramirez VD, 1982. Luteinizing hormone-releasing hormone release during the rat estrous cycle and after ovariectomy, as estimated with push-pull cannulae. Endocrinology 111:1439–1448PubMedCrossRefGoogle Scholar
  3. Mcintosh J, Mcintosh RP, 1986. Varying the patterns and concentrations of GnRH stimulation does not alter the ratio of LH and FSH released from perifused sheep pituitary cells. J Endocrinol 109:155–161PubMedCrossRefGoogle Scholar
  4. Morrison DF, 1967. Multivariate Statistical Methods. New York:McGraw-Hill, pp. 141–148Google Scholar
  5. Sarkar DK, Chiappa SA, Fink G, Sherwood NM, 1976. Gonadotropin-releasing hormone surge in pro-oestrous rats. Nature 264:461–463PubMedCrossRefGoogle Scholar
  6. Savoy-Moore RT, Landefeld T, Marshall JC, 1980. Hormonal measurement in rat anterior pituitary cell cultures: loss of immunoreactive LH counteracted by fetal calf serum and bacitracin. Mol Cell Endocrinol 18:11–20PubMedCrossRefGoogle Scholar
  7. Savoy-Moore RT, Swartz KH, 1986. Isolated, perfused rat anterior pituitary cells can differentially release FSH following GnRH stimulation. Abstract 128, Society for the Study of Reproduction 19th Annual MeetingGoogle Scholar
  8. Smith M, Freeman M, Neill J, 1975. The control of progesterone secretion during the estrous cycle and early pseudopregnancy in the rat: prolactin, gonadotropin and steroid levels associated with rescue of the corpus luteum of pseudopregnancy. Endocrinology 96:219–226PubMedCrossRefGoogle Scholar
  9. Wildt L, Hausier A, Marshall G, Hutchison JS, Plant TM, Belchetz PE, Knobil E, 1981. Frequency and amplitude of GnRH stimulation and gonadotropin secretion in the Rhesus monkey. Endocrinology 109:376–385PubMedCrossRefGoogle Scholar
  10. Wise PM, Rance N, Barr GD, Barraclough CA, 1979. Further evidence that luteinizing hormone-releasing hormone also is follicle-stimulating hormone-releasing hormone. Endocrinology 104:940–947PubMedCrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1987

Authors and Affiliations

  • R. T. Savoy-Moore
    • 1
  • K. H. Swartz
    • 1
  1. 1.Departments of OB/GYN and PhysiologyWayne State UniversityDetroitUSA

Personalised recommendations