The Effects of Low O2 and Antioxidants on Steroidogenic Capacity in Cultured Rat Leydig Cells
Part of the
Advances in Experimental Medicine and Biology
book series (AEMB, volume 219)
The development of methods for Leydig cell culture in a chemically defined medium has proven of considerable value to numerous investigators in recent years. It is now possible to study hormone action and control of Leydig cell steroidogenesis using such a system. Most investigators report that mature rat Leydig cells in culture remain viable for 7–10 days, however, function (LH/hCG binding to membrane receptors and testosterone production) is lost after 3–4 days (Mather et al., 1981; Browning et al., 1983).
KeywordsLeydig Cell Testosterone Production Progesterone Production Cholesterol Side Chain Cleavage Mitochondrial Cholesterol
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© Plenum Press, New York 1987