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The Effects of Low O2 and Antioxidants on Steroidogenic Capacity in Cultured Rat Leydig Cells

  • T. O. Abney
  • R. B. Meyers
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 219)

Abstract

The development of methods for Leydig cell culture in a chemically defined medium has proven of considerable value to numerous investigators in recent years. It is now possible to study hormone action and control of Leydig cell steroidogenesis using such a system. Most investigators report that mature rat Leydig cells in culture remain viable for 7–10 days, however, function (LH/hCG binding to membrane receptors and testosterone production) is lost after 3–4 days (Mather et al., 1981; Browning et al., 1983).

Keywords

Leydig Cell Testosterone Production Progesterone Production Cholesterol Side Chain Cleavage Mitochondrial Cholesterol 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Browning JY, Heindel JJ, Grotjan HE, 1983. Primary culture of purified Leydig cells isolated from adult rat testes. Endocrinology 112:543–549PubMedCrossRefGoogle Scholar
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Copyright information

© Plenum Press, New York 1987

Authors and Affiliations

  • T. O. Abney
    • 1
  • R. B. Meyers
    • 1
  1. 1.Department of Physiology and EndocrinologyMedical College of GeorgiaAugustaUSA

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