Chronic Effects of Insulin and Insulin-Like Growth Factor-1 on Steroidogenesis by Rat Leydig Cells

  • William H. Moger
  • Paul R. Murphy
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 219)


There are no apparent acute effects of insulin on Leydig cell glucose transport or androgen production (Moger and Murphy, 1986). However, chronic exposure of primary cultures of rat or mouse testicular cells to insulin has been reported to increase LH-stimulated androgen production (Adashi et al., 1982; Murphy and Moger, 1982). Insulin-like growth factor-1 (IGF-1) receptors have been reported to be present on Leydig cells (Handelsman et al., 1985; Lin et al., 1986a) and recent reports have suggested an intratesticular site of IGF-1 production (D’Ercole et al., 1984; Handelsman et al., 1985). As with insulin, IGF-1 did not acutely alter androgen production by Leydig cells (Handelsman et al., 1985). The present study has compared the chronic effects of insulin and IGF-1 on androgen production by purified rat Leydig cells.


Leydig Cell Androgen Production Androgen Secretion Leydig Cell Steroidogenesis Concentration Dependent Fashion 
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  1. Adashi EY, Fabics C, Hsueh AJW, 1982. Insulin augmentation of testosterone production in a primary culture of rat testicular cells. Biol Reprod 26:270–280PubMedCrossRefGoogle Scholar
  2. Anakwe OO, Moger WH, 1986. Catecholamine stimulation of androgen production by rat Leydig cells. Interactions with luteinizing hormone and luteinizing hormone-releasing hormone. Biol Reprod In PressGoogle Scholar
  3. DeLean A, Munson PJ, Rodbard D, 1978. Simulatneous analysis of families of sigmoidal curves; application to bioassay, radio-ligand assay and physiological dose-response curves. Am J Physiol 235:E97–E102PubMedGoogle Scholar
  4. D’Ercole AJ, Stiles AD, Underwood LE, 1984. Tissue concentrations of somatomedian C: further evidence for multiple sites of synthesis and paracrine or autocrine mechanisms of action. Proc Natl Acad Sci USA 81:935–939PubMedCrossRefGoogle Scholar
  5. Froesch ER, Schmid C, Schwander J, Zapf J, 1985. Actions of insulin-like growth factors. Ann Rev Physiol 47:443–467CrossRefGoogle Scholar
  6. Handelsman DJ, Spaliviero JA, Scott CD, Baxter RC, 1985. Identification of insulin-like growth factor-1 and its receptors in the rat testis. Acta Endocr 109:543–549PubMedGoogle Scholar
  7. Lin T, Haskell J, Vinson N, Terracio L, 1986a. Direct stimulatory effects of insulin-like growth factor-I on Leydig cell steroidogenesis in primary culture. Biochem Biophys Res Comm 137:950–956PubMedCrossRefGoogle Scholar
  8. Lin T, Haskell J, Vinson N, Terracio L, 1986b. Characterization of insulin and insulin-like growth factor I receptors of purified Leydig cells and their role in steroidogenesis in primary culture: a comparative study. Endocrinology 119:1641–1647PubMedCrossRefGoogle Scholar
  9. Moger WH, Murphy PR, 1986. Fuel requirements for Leydig cell steroidogenesis. Prog Soc Obst Gyn Canada p. 126Google Scholar
  10. Murphy PR, Moger WH, 1982. Short-term primary culture of mouse interstitial cells: effects of culture conditions on androgen production. Bio Reprod 27:38–47CrossRefGoogle Scholar
  11. West DC, Sattar A, Kumar S, 1985. A simplified in situ solubilization procedure for the determination of DNA and cell number in cultured mammalian cells. Anal Biochem 147:289–295PubMedCrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1987

Authors and Affiliations

  • William H. Moger
    • 1
    • 2
  • Paul R. Murphy
    • 3
  1. 1.Departments of Physiology and BiophysicsDalhousie UniversityHalifaxCanada
  2. 2.Departments of Obstetrics and GynecologyDalhousie UniversityHalifaxCanada
  3. 3.Department of PhysiologyUniversity of ManitobaWinnipegCanada

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