Spermatogenesis In Vitro: Searching for In Vivo Correlates
The development of methodology for the study of spermatogenesis in vitro has been a major task that remained elusive (Wolff and Haffen, 1965; Steinberger, 1975). Significant advances have been reported in the culture of meiotic cells of lilaceous plants (Hotta et al., 1966), and spermatogenic cells of Xenopus (Risley, 1983) and Drosophila (Liebrich, 1981). Attempts to maintain isolated spermatogenic cells viable for long periods of time (more than 48 h) have been relatively unsuccessful. In fact, the seminiferous epithelium contains spermatogenic cells that maintain spatial and functional relationships with Sertoli cells, and this cell-cell interaction is likely to play an important role in spermatogenesis in vivo and in vitro.
KeywordsSertoli Cell Seminiferous Tubule Spermatogenic Cell Seminiferous Epithelium Pachytene Spermatocyte
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