Demonstration of hCG Binding Sites and hCG Stimulated Steroidogenesis in Different Populations of Interstitial Cells

  • V. K. Bhalla
  • E. S. Browne
  • G. S. Sohal
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 219)


To gain further knowledge of the mechanism of gonadotropin action in testes, a number of cell purification methods have been employed to isolate the morphologically distinct and biochemically responsive Leydig cells from the variety of cell types found in the testicular interstitium. Centrifugal fractionation of collagenase dispersed cells on density gradients of Metrizamide has been proven to be of considerable value (Conn et al., 1977; Dehejia et al., 1982). Typical Leydig cells were identified in a cell band (density 1.105 g/cm3) which contained a large number of receptors for LH/hCG and responded to the hormone in vitro by producing cAMP and testosterone. A lighter cell fraction (density 1.085 g/cm3) showed cells with a distinct morphology but little hCG binding or steroidogenic response. Similar observations were also reported in mouse testes (Schumacher et al., 1978). Payne et al. (1980a, b), on the other hand, suggested the existence of two distinct populations of hCG responsive testicular interstitial cells. The cells in population I (density 1.085–1.117 g/cm3) produced less testosterone in response to hCG than cells in population II (density 1.128–1.148 g/cm3). The binding affinity for hCG in both populations was, however, the same. All of these observations characterized typical Leydig cells as cells that exhibit high affinity binding of LH/hCG and produce testosterone in response to the hormone.


Leydig Cell Interstitial Cell Luteinizing Hormone Receptor Testosterone Production High Affinity Binding Site 
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Copyright information

© Plenum Press, New York 1987

Authors and Affiliations

  • V. K. Bhalla
    • 1
  • E. S. Browne
    • 1
  • G. S. Sohal
    • 2
  1. 1.Department of Physiology and EndocrinologyMedical College of GeorgiaAugustaUSA
  2. 2.Department of AnatomyMedical College of GeorgiaAugustaUSA

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