Regulation of Mitochondrial and Microsomal Cytochrome P-450 Enzymes in Leydig Cells

  • Anita H. Payne
  • Onyeama O. Anakwe
  • Dale B. Hales
  • Markos Georgiou
  • Louise M. Perkins
  • Patrick G. Quinn
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 219)


Testosterone biosynthesis in Leydig cells is dependent on both acute and chronic stimulation by the anterior pituitary hormone, luteinizing hormone (LH). Binding of LH to specific high-affinity receptors on the surface of Leydig cells (Catt et al., 1972) results in increased production of intracellular cAMP (Dufau et al., 1973). Both the acute and the chronic effects of LH are mediated by increases in cyclic AMP. A schematic representation of LH stimulated testosterone production in Leydig cells is shown in Fig. 1. Although the exact mechanism is not known, acute stimulation by trophic hormones or cAMP in all steroidogenic tissues (testis, ovary and adrenal) results in an increased amount of the endogenous precursor, cholesterol, which associates with the mitochondrial cholesterol side-chain cleavage enzyme (P-450SCC). Chronic treatment of intact or hypophysectomized rats with LH, or its analog, human chorionic gonadotropin (hCG), results in an increased capacity for LH-stimulated testosterone production (Zipf et al., 1978; Payne et al., 1980) and induces enzymes of the steroidogenic pathway (Purvis et al., 1973a, b; O’Shaughnessy and Payne, 1982).


Luteinizing Hormone Leydig Cell Luteinizing Hormone Receptor Testosterone Production Steroid Product 
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Copyright information

© Plenum Press, New York 1987

Authors and Affiliations

  • Anita H. Payne
    • 1
  • Onyeama O. Anakwe
    • 1
  • Dale B. Hales
    • 1
  • Markos Georgiou
    • 1
  • Louise M. Perkins
    • 1
  • Patrick G. Quinn
    • 1
  1. 1.Departments of Obstetrics and Gynecology, Biological Chemistry and The Reproductive Endocrinology ProgramThe University of MichiganAnn ArborUSA

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