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A Two-Dimensional NMR Study of the Complex of lac Repressor Headpiece with a 14 Base Pair lac Operator Fragment

  • R. Boelens
  • R. M. Scheek
  • R. M. J. N. Lamerichs
  • J. de Vlieg
  • J. H. van Boom
  • R. Kaptein
Part of the NATO ASI Series book series (volume 137)

Abstract

Lac repressor of E. coli recognizes a 20 to 25 basepair DNA sequence called the lac operator.1–4 When it binds to this region it blocks transcription of the genes coding for the lactose enzymes. Genetic and biochemical data have implicated the N-terminal part or ‘headpiece’ of the repressor as the DNA binding domain.4, 5 The precise way in which lac repressor recognizes its operator is, however, not known.

Keywords

Nuclear Magnetic Resonance Nuclear Magnetic Resonance Spectrum Cross Peak Base Proton Recognition Helix 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Plenum Press, New York 1987

Authors and Affiliations

  • R. Boelens
    • 1
  • R. M. Scheek
    • 1
  • R. M. J. N. Lamerichs
    • 1
  • J. de Vlieg
    • 1
  • J. H. van Boom
    • 2
  • R. Kaptein
    • 1
  1. 1.Laboratory of Physical ChemistryUniversity of GroningenThe Netherlands
  2. 2.Gorlaeus LaboratoryUniversity of LeidenThe Netherlands

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