Advertisement

Use of Gene Transfer in the Isolation of Cell Surface Receptor Genes

  • Dan R. Littman
  • Moses V. Chao
Part of the Genetic Engineering book series (GEPM)

Abstract

The study of cell surface receptors is essential for understanding means by which cells communicate with each other. In recent years, there has been a tremendous surge of interest in such molecules, in large part because several of them have been shown to be involved in regulation of cell growth. In particular, both the EGF and CSF-1 receptors have been shown to have oncogenic potential when expressed in altered form as viral oncogenes (1,2). Numerous cellular genes which encode cell surface receptors have been recently cloned, and studies using these genes are yielding valuable insight into mechanisms of cell activation and of cell-cell interaction. Most receptor genes have been isolated by conventional means involving protein purification and subsequent sequence determination. The receptors for epidermal growth factor (1), insulin (3), platelet-derived growth factor (4), and interleukin-2 (5) have been subjected to this approach. Other approaches have depended on enrichment of mRNA encoding the receptor; the low density lipoprotein receptor (6) and the T cell receptor (7) genes have been isolated by such means. We have employed an alternative approach which relies on DNA mediated gene transfer into mammalian cells and detection of the transfected gene product (Figure 1).

Keywords

Single Copy Gene Herpes Simplex Thymidine Kinase Gene Double Minute Chromosome Rosetting Assay Transferrin Receptor Gene 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Ullrich, A., Coussens, L., Hayflick, J.S., Dull, T.J., Gray, A., Tam, A.W., Lee, J., Yarden, Y., Libermann, T.A., Schlessinger, J., Downward, J., Mayes, E.L.V., Whittle, N., Waterfield, M.D. and Seeburg, P.H. (1984) Nature 309, 418–429.CrossRefGoogle Scholar
  2. 2.
    Sherr, C.J., Rettenmier, C.W., Sacca, R., Roussel, M.F., Look, A.T. and Stanley, E.R. (1985) Cell 41, 665–676.CrossRefGoogle Scholar
  3. 3.
    Ebina, Y., Edery, M., Ellis, L., Standring, D., Beaudoin, J., Roth, R.A. and Rutter, W.J. (1985) Proc. Nat. Acad. Sci. U.S.A. 82, 8014–8018.CrossRefGoogle Scholar
  4. 4.
    Yarden, Y., Escobedo, J.A., Kuang, W.-J., Yang-Feng, T.L., Daniel, T.O., Tremble, P.M., Chen, E.Y., Ando, M.E., Harkins, R.N., Francke, U., Fried, V.A., Ullrich, A. and Williams, L.T. (1986) Nature 323, 226–232.CrossRefGoogle Scholar
  5. 5.
    Leonard, W.J., Depper, J.M., Crabtree, G.R., Rudikoff, S., Humphrey, J., Robb, R.J., Knonke, M., Svetlik, P.B., Peffer, N.J., Waldmann, T. and Greene, W.C. (1984) Nature 311, 626–631.CrossRefGoogle Scholar
  6. 6.
    Russell, D.W., Yamamoto, T., Schneider, W.J., Slaughter, C.J., Brown, M.S. and Goldstein, J.L. (1983) Proc. Nat. Acad. Sci. U.S.A. 80, 7501–7505.CrossRefGoogle Scholar
  7. 7.
    Hedrick, S.M., Cohen, D.I., Nielsen, E.A. and Davis, M.M. (1984) Nature 308, 149–153.CrossRefGoogle Scholar
  8. 8.
    Littman, D.R., Thomas, Y., Maddon, P.J., Chess, L. and Axel, R. (1985) Cell 40, 237–246.CrossRefGoogle Scholar
  9. 9.
    Maddon, P.J., Littman, D.R., Godfrey, M., Maddon, D.E., Chess, L. and Axel, R. (1985) Cell 42, 93–104.CrossRefGoogle Scholar
  10. 10.
    Chao, M.V., Bothwell, M.A., Ross, A.H., Koprowski, H., Lanahan, A., Buck, C.R. and Sehgal, A. (1986) Science 232, 518–521.CrossRefGoogle Scholar
  11. 11.
    Graham, F.L. and Van der Eb, A.J. (1973) Virology 52, 456–467.CrossRefGoogle Scholar
  12. 12.
    Wigler, M., Pellicer, A., Silverstein, S. and Axel, R. (1978) Cell 14, 725–731.CrossRefGoogle Scholar
  13. 13.
    Jolly, D.J., Esty, A.C., Bernard, H.U. and Friedmann, T. (1982) Proc. Nat. Acad. Sci. U.S.A. 79, 5038–5041.CrossRefGoogle Scholar
  14. 14.
    Lowy, I., Pellicer, A., Jackson, J.F., Sim, G.K., Silverstein, S. and Axel, R. (1980) Cell 22, 817–823.CrossRefGoogle Scholar
  15. 15.
    Wigler, M., Perucho, M., Kurtz, D., Dana, S., Pellicer, A., Axel, R. and Silverstein, S. (1980) Proc. Nat. Acad. Sci. U.S.A. 77, 3567–3570.CrossRefGoogle Scholar
  16. 16.
    Shih, C. and Weinberg, R.A. (1982) Cell 29, 161–169.CrossRefGoogle Scholar
  17. 17.
    Wigler, M., Sweet, R., Sim, G.K., Wold, B., Pellicer, A., Lacy, E., Maniatis, T., Silverstein, S. and Axel, R. (1979) Cell 16, 777–785.CrossRefGoogle Scholar
  18. 18.
    Hsu, C., Kavathas, P. and Herzenberg, L.A. (1984) Nature 312, 68–69.CrossRefGoogle Scholar
  19. 19.
    Stein, R., Razin, A. and Cedar, H. (1982) Proc. Nat. Acad. Sci. U.S.A. 79, 3418–3422.CrossRefGoogle Scholar
  20. 20.
    Busslinger, M., Hurst, J. and Flavell, R. (1983) Cell 34, 197–206.CrossRefGoogle Scholar
  21. 21.
    Kavathas, P. and Herzenberg, L.A. (1983) Proc. Nat. Acad. Sci. U.S.A. 80, 524–528.CrossRefGoogle Scholar
  22. 22.
    Kuhn, L.C., McClelland, A. and Ruddle, F.H. (1984) Cell 37, 95–103.CrossRefGoogle Scholar
  23. 23.
    Kavathas, P. and Herzenberg, L.A. (1983) Nature 306, 385–387.CrossRefGoogle Scholar
  24. 24.
    Goding, J.W. (1976) J. Immunol. Methods 10, 61–66.CrossRefGoogle Scholar
  25. 25.
    Mendelsohn, C., Johnson, B., Lionetti, K.A., Nobis, P., Wimmer, E. and Racaniello, V.R. (1986) Proc. Nat. Acad. Sci. U.S.A. 83, 7845–7849.CrossRefGoogle Scholar
  26. 26.
    Albino, A.P., Graf, L.H., Kantor, R.R.S., McLean, W., Silagi, S. and Old, L. (1985) Mol. Cell. Biol. 5, 692–697.Google Scholar
  27. 27.
    Wysoki, L.J. and Sato, V.L. (1978) Proc. Nat. Acad. Sci. U.S.A. 75, 2844–2847.CrossRefGoogle Scholar
  28. 28.
    Maryarisky, J.L., DePlaen, E. and VanSnick, J. (1985) J. Immunol. Methods 79, 159–165.CrossRefGoogle Scholar
  29. 29.
    Berman, J.W., Basch, R.S. and Pellicer, A. (1984) Proc. Nat. Acad. Sci. U.S.A. 81, 7176–7179.CrossRefGoogle Scholar
  30. 30.
    Jelinek, W.R., Roomey, T.P., Leinwand, L., Duncan, C.H., Biro, P.M., Choudary, P.V., Weissman, S.M., Rubin, C.M., Houck, C.M., Deininger, P.L. and Schmid, C.W. (1980) Proc. Nat. Acad. Sci. U.S.A. 77, 1398–1402.CrossRefGoogle Scholar
  31. 31.
    Perucho, M., Hanahan, D., Lipsich, L. and Wigler, M. (1980) Nature 285, 207–210.CrossRefGoogle Scholar
  32. 32.
    Goldfarb, M., Shimizu, K., Perucho, M. and Wigler, M. (1982) Nature 296, 404–409.CrossRefGoogle Scholar
  33. 33.
    Kavathas, P., Sukhatme, V.P., Herzenberg, L.A. and Parnes, J.R. (1984) Proc. Nat. Acad. Sci. U.S.A. 81, 7688–7692.CrossRefGoogle Scholar
  34. 34.
    Davis, M.M., Cohen, D.I., Nielsen, E.A., Steinmetz, M., Paul, W.E. and Hood, L. (1984) Proc. Nat. Acad. Sci. U.S.A. 81, 2194–2198.CrossRefGoogle Scholar
  35. 35.
    Okayama, H. and Berg, P. (1983) Mol. Cell. Biol. 3: 280–289.Google Scholar
  36. 36.
    Hiraki, D.D., Nomura, D., Yokota, T., Arai, K.-I. and Coffman, R.L. (1986) J. Immunol. 136, 4291–4296.Google Scholar
  37. 37.
    Cepko, C., Roberts, B. and Mulligan, R.C. (1984) Cell 37, 1053–1062.CrossRefGoogle Scholar
  38. 38.
    Cosgrove, L.H., Sandrin, M.S., Rajasekariah, P. and McKenzie, I.F.C. (1986) Proc. Nat. Acad. Sci. U.S.A. 83, 752–756.CrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1987

Authors and Affiliations

  • Dan R. Littman
    • 1
  • Moses V. Chao
    • 2
  1. 1.Department of Microbiology and ImmunologyUniversity of California, San Francisco School of MedicineSan FranciscoUSA
  2. 2.Department of Cell Biology and AnatomyCornell University Medical SchoolNew YorkUSA

Personalised recommendations