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DNase I Footprinting as an Assay for Mammalian Gene Regulatory Proteins

  • William S. Dynan
Part of the Genetic Engineering book series (GEPM)

Abstract

The DNase I footprinting method was first described by Galas and Schmitz (1). The technique remains unsurpassed as a way of gaining direct and immediate information about the location of a protein binding site in the DNA sequence. In addition, the footprinting method provides a way of studying specific binding in the presence of a high nonspecific background, a common situation when working with rare proteins that have been only partially purified. So effective is the footprinting method in discriminating between specific and nonspecific binding that it is often possible to visualize specific protein-DNA interactions with entirely unfractionated protein extracts from mammalian nuclei.

Keywords

Packed Cell Volume Hypotonic Lysis Buffer Footprinting Method Rare Protein DNase Footprinting 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Plenum Press, New York 1987

Authors and Affiliations

  • William S. Dynan
    • 1
  1. 1.Department of Chemistry and BiochemistryUniversity of ColoradoBoulderUSA

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