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The Expression of Introduced Genes in Regenerated Plants

  • Pamela Dunsmuir
  • John Bedbrook
  • Diane Bond-Nutter
  • Caroline Dean
  • David Gidoni
  • Jonathan Jones
Part of the Genetic Engineering book series (GEPM)

Abstract

The transfer of foreign genes into plant cells has become a routine and commonly used technique. Most frequently the method relies upon the natural gene transfer system from Agrobacterium tumefaciens or modifications thereof (1,2). However, the recently developed system of protoplast electroporation is also being used as an effective method of gene transfer to both dicotyledonous and monocotyledonous species (3,4). There is a recent excellent review which thoroughly describes the most efficient methods, and the systems available for gene transfer into plant cells, and summarizes the use of gene transfer as a tool for studying plant gene expression (5). Here we will describe data which we have accumulated on the expression of genes introduced into plants, and we will briefly summarize similar data from other laboratories. The overall perspective of this review is to evaluate the issues of introduced gene expression relevant to using the technology to develop improved plant varieties.

Keywords

Regenerate Plant Foreign Gene Promoter Fragment Chloramphenicol Acetyl Transferase Independent Transformants 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Plenum Press, New York 1987

Authors and Affiliations

  • Pamela Dunsmuir
    • 1
  • John Bedbrook
    • 1
  • Diane Bond-Nutter
    • 1
  • Caroline Dean
    • 1
  • David Gidoni
    • 1
  • Jonathan Jones
    • 1
  1. 1.Advanced Genetic Sciences, Inc.OaklandUSA

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