The Expression of Introduced Genes in Regenerated Plants

  • Pamela Dunsmuir
  • John Bedbrook
  • Diane Bond-Nutter
  • Caroline Dean
  • David Gidoni
  • Jonathan Jones
Part of the Genetic Engineering book series (GEPM)


The transfer of foreign genes into plant cells has become a routine and commonly used technique. Most frequently the method relies upon the natural gene transfer system from Agrobacterium tumefaciens or modifications thereof (1,2). However, the recently developed system of protoplast electroporation is also being used as an effective method of gene transfer to both dicotyledonous and monocotyledonous species (3,4). There is a recent excellent review which thoroughly describes the most efficient methods, and the systems available for gene transfer into plant cells, and summarizes the use of gene transfer as a tool for studying plant gene expression (5). Here we will describe data which we have accumulated on the expression of genes introduced into plants, and we will briefly summarize similar data from other laboratories. The overall perspective of this review is to evaluate the issues of introduced gene expression relevant to using the technology to develop improved plant varieties.


Regenerate Plant Foreign Gene Promoter Fragment Chloramphenicol Acetyl Transferase Independent Transformants 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Chilton, M., Drummond, M., Merlo, D., Sciaky, D., Montoya, A., Gordon, M. and Nester, E. (1977) Cell 11, 263–271.CrossRefGoogle Scholar
  2. 2.
    Schell, J. and Van Montagu, M. (1983) Bio/Technology 1, 175–181.CrossRefGoogle Scholar
  3. 3.
    Potrykus, I., Shilloto, R. and Saul, M. (1985) Mol. Gen. Genet. 199, 183–188.CrossRefGoogle Scholar
  4. 4.
    Fromm, M., Taylor, L. and Walbot, V. (1985) Proc. Nat. Acad. Sci. U.S.A., 82, 5824–5828.CrossRefGoogle Scholar
  5. 5.
    Fraley, R., Rogers, S. and Horsch, R. (1986) CRC Crit. Rev. Plant. Sci. 4, 1–46.CrossRefGoogle Scholar
  6. 6.
    Dunsmuir, P., Smith, S. and Bedbrook. J. (1983) J. Mol. Appl. Gen. 2, 285–300.Google Scholar
  7. 7.
    van den Elzen, P., Lee, K., Townsend, J. and Bedbrook, (1985) J. Plant Mol. Biol. 5, 149–154.CrossRefGoogle Scholar
  8. 8.
    Maliga, P., Breznovits, A. and Marton, L. (1973) Nature 244, 29–30.Google Scholar
  9. 9.
    McKnight, S. (1982) Cell 31, 355–365.CrossRefGoogle Scholar
  10. 10.
    Murashige, T. and Skoog, F. (1962) Physiol. Plant 15, 473–497.CrossRefGoogle Scholar
  11. 11.
    Dean, C., van den Elzen, P., Tamaki, S., Dunsmuir, P. and Bedbrook, J. (1985) Proc. Nat. Acad. Sci. U.S.A. 82, 4964–4968.CrossRefGoogle Scholar
  12. 12.
    Dean, C., van den Elzen, P., Tamaki, S., Dunsmuir, P. and Bedbrook, J. (1985) EMBO J. 4, 3055–3061.Google Scholar
  13. 13.
    Nagy, F., Kay, S.A. Boutry, M., Hsu, M.-Y. and Chua, N.-H. (1986) EMBO J. 5, 1119–1124.Google Scholar
  14. 14.
    Lamppa, G., Nagy, F. and Chua, N.-H. (1985) Nature 316, 750–752.CrossRefGoogle Scholar
  15. 15.
    Eckes, P., Rosahl, S., Schell, J. and Willmitzer, L. (1986) Mol. Gen. Genet, (in press).Google Scholar
  16. 16.
    Jones, J., Dunsmuir, P. and Bedbrook, J. (1985) EMBO J. 4, 2411–2418.Google Scholar
  17. 17.
    Simpson, J., Van Montagu, M. and Herrera-Estrella, L. (1986) Science 233, 34–38.CrossRefGoogle Scholar
  18. 18.
    Hall, T., Reichert, N., Sengupta-Gopalan, C., Cramer, J., Lea, C., Barker, R., Slightom, J., Klassey, R. and Kemp, J. (1985) in Molecular Form and Function of the Plant Genome (Vloten-Doting, E.L., Groot, G. and Hall, T., eds.), pp. 517–530, Plenum Press, New York, NY.Google Scholar
  19. 19.
    Ellis, J., Llewellyn, D., Dennis, E. and Peacock, J. (1986) EMBO J. (submitted).Google Scholar
  20. 20.
    Beachy, R.N., Chen, Z.-L., Horsch, R.B., Rogers, S.G., Hoffman, N.J. and Fraley, R.T. (1985) EMBO J. 4, 3047–3053.Google Scholar
  21. 21.
    Sengupta-Gopalan, C., Reichert, N., Barker, R., Hall, T. and Kemp, J. (1985) Proc. Nat. Acad. Sci. U.S.A. 82, 3320–3324.CrossRefGoogle Scholar
  22. 22.
    Chen, Z., Schuler, M. and Beachy, R. (1986) Proc. Nat. Acad. Sci. U.S.A. (in press).Google Scholar
  23. 23.
    Nagy, F., Morelli, G., Fraley, R., Rogers, S. and Chua, N. (1985) EMBO J. 4, 3063–3068.Google Scholar
  24. 24.
    Fluhr, R., Kuhlemeir, C., Nagy, N. and Chua, N. (1986) Science 232, 1106–1112CrossRefGoogle Scholar
  25. 25.
    Dunsmuir, P. (1985) Nucl. Acids Res. 13, 2503–2518.CrossRefGoogle Scholar
  26. 26.
    Bevan, M., Barnes, W. and Chilton, M. (1983) Nucl. Acids Res. 11, 369–385.CrossRefGoogle Scholar
  27. 27.
    Depicker, A., Stachel, S., Dhaese, P., Zambryski, P. and Goodman, H. (1985) J. Mol. Appl. Genet. 1, 561.Google Scholar
  28. 28.
    Jones, J.D.G., Grady, K.L., Suslow, T.V., and Bedbrook, J.R. (1986) EMBO J. 5, 467–473.Google Scholar
  29. 29.
    Alton, N. and Vapnek, D. (1979) Nature 282, 864–869.CrossRefGoogle Scholar
  30. 30.
    Kozak, M. (1986) Cell 44, 283–292.CrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1987

Authors and Affiliations

  • Pamela Dunsmuir
    • 1
  • John Bedbrook
    • 1
  • Diane Bond-Nutter
    • 1
  • Caroline Dean
    • 1
  • David Gidoni
    • 1
  • Jonathan Jones
    • 1
  1. 1.Advanced Genetic Sciences, Inc.OaklandUSA

Personalised recommendations