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Gene Transfer in the Sea Urchin

  • Barbara R. Hough-Evans
  • Eric H. Davidson
Part of the Genetic Engineering book series (GEPM)

Abstract

Sea urchins began to be used over a century ago for studies of fertilization and development, and they are now among the best understood experimental models for early embryogenesis. The use of sea urchin embryos can be attributed in part to practical considerations. The adult animals are abundant, widely distributed, and easy to collect. Gametes are available in relatively large quantities during long breeding seasons, and under appropriate laboratory regimens they can be obtained all year round (1,2). Embryonic development is rapid and synchronous, occurs reliably under laboratory conditions, and in most species is complete within a few days. Unlike most invertebrates commonly utilized for research, echinoderms belong to the same great branch of the Animal Kingdom as do the vertebrates, i.e., and they are deuterostomes. Thus to the advantage of experimental accessibility may be added the attraction of a developmental system that shares with the chordates a common if remote evolutionary ancestry. Definitive homologies in the morphogenesis of chordate and echinoderm embryos were noted by classical observers [reviewed in (3)], and in recent years specific molecular homologies have been reported as well.

Keywords

Chloramphenicol Acetyl Transferase Chloramphenicol Acetyl Transferase Activity Simplex Virus Thymidine Kinase Gene Pluteus Stage Exogenous Sequence 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. 1.
    Leahy, P.S. and Hough-Evans, B.R., Britten, R.J. and Davidson, E.H. (1981) J. Exp. Zool. 215, 7–22.CrossRefGoogle Scholar
  2. 2.
    Leahy, P.S. and Tutschulte, T.C. and Britten, R.J. and Davidson, E.H. (1978) J. Exp. Zool. 204, 369–380.CrossRefGoogle Scholar
  3. 3.
    Hyman, L.H. (1955) The Invertebrates: Echinodermata. The Coelomate Bilateria, Vol. IV, McGraw-Hill, New York, NY.Google Scholar
  4. 4.
    Carroll, S.L. and Bergsma, D.J. and Schwartz, R.J. (1986) J. Biol. Chem. 261, 8965–8976.Google Scholar
  5. 5.
    Fornwald, J.A. and Kuncio, G., Peng, I. and Ordahl, C.P. (1982) Nucl. Acids. Res. 10, 3861–3876.CrossRefGoogle Scholar
  6. 6.
    Zakut, R. and Shani, M., Givol, D. and Neuman, S., Yaffe, D. and Nudel, U. (1982) Nature 298, 857–859.CrossRefGoogle Scholar
  7. 7.
    Davidson, E.H. and Thomas, T.L. and Scheller, R.H. and Britten, R.J. (1982) in Genome Evolution (Dover, G.A. and Flavell, R.B., eds.) pp. 177–191, Academic Press, London.Google Scholar
  8. 8.
    Hörstadius, S. (1938) Biol. Rev. Cambridge Philos. Soc. 14, 132–179.CrossRefGoogle Scholar
  9. 9.
    Hörstadius, S. (1973) Experimental Embryology of Echinoderms, Clarendon Press, Oxford.Google Scholar
  10. 10.
    Czihak, G. (1971) in Experimental Embryology of Marine and Fresh Water Invertebrates (Reverberi, G. and ed.) pp. 363–506, North Holland, Amsterdam.Google Scholar
  11. 11.
    Giudice, G. (1973) Developmental Biology of the Sea Urchin Embryo, Academic Press, New York, NY.Google Scholar
  12. 12.
    Davidson, E.H. (1986) Gene Activity in Early Development, Third Edition, Academic Press, Orlando, Florida.Google Scholar
  13. 13.
    Cameron, R.A. and Hough-Evans, B.R. and Britten, R.J. and Davidson, E.H. (1986) Dev. Biol. and submitted for publicationGoogle Scholar
  14. 14.
    MacBride, E.W. (1903) Phil. Trans. Roy. Soc. London B 195, 285–327.CrossRefGoogle Scholar
  15. 15.
    Cameron, R.A. and Hinegardner, R.T. (1974) Biol. Bull. 146, 335–342.CrossRefGoogle Scholar
  16. 16.
    Flytzanis, C.N. McMahon, A.P., Hough-Evans, B.R. and Katula, K.S. and Britten, R.J. and Davidson, E.H. (1985) Dev. Biol. 108, 431–442.CrossRefGoogle Scholar
  17. 17.
    Davidson, E.H. and Hough-Evans, B.R. and Britten, R.J. (1982) Science 217, 17–26.CrossRefGoogle Scholar
  18. 18.
    Hentschei, C.C. and Birnstiel, M.L. (1981) Celi 25, 301–313.CrossRefGoogle Scholar
  19. 19.
    Nemer, M. and Travaglini, E.C. and Rondinelli, E. and D’Alonzo, J. (1984) Dev. Biol. 102, 471–482.CrossRefGoogle Scholar
  20. 20.
    Nemer, M. and Wilkinson, D.G. and Travaglini, E.C. (1985) Dev. Biol. 109, 418–427.CrossRefGoogle Scholar
  21. 21.
    Alexandraki, D. and Ruderman, J.V. (1985) Dev. Biol. 109, 436–451.CrossRefGoogle Scholar
  22. 22.
    Shott, R.J. and Lee, J.J., Britten, R.J. and Davidson, E.H. (1984) Dev. Biol. 101, 295–306.CrossRefGoogle Scholar
  23. 23.
    Lee, J.J. and Calzone, F.J., Britten, R.J. and Angerer, R.C. and Davidson, E.H. (1986) J. Mol. Biol. 188, 173–183.CrossRefGoogle Scholar
  24. 24.
    Cox, K.H. and Angerer, L.M., Lee, J.J., Davidson, E.H. and Angerer, R.C. (1985) J. Mol. Biol. 188, 159–172.CrossRefGoogle Scholar
  25. 25.
    Durica, D.S. and Crain, W.R. (1982) Dev. Biol. 92, 428–439.CrossRefGoogle Scholar
  26. 26.
    Lynn, D.A. and Angerer, L.M., Bruskin, A.M., Klein, W.H. and Angerer, R.C. (1983) Proc. Nat. Acad. Sci. U.S.A. 80, 2656–2660.CrossRefGoogle Scholar
  27. 27.
    Bruskin, A.M. and Bedard, P.A. and Tyner, A.L. and Showman, R.M. and Brandhorst, B.P. and Klein, W.H. (1982) Dev. Biol. 91, 317–324.CrossRefGoogle Scholar
  28. 28.
    Benson, S. and Sucov, H.M. and Stephens, L. and Davidson, E.H. and Wilt, F. (1986) Dev. Biol, submitted for publication.Google Scholar
  29. 29.
    Sucov, H.M. and Benson, S. and Britten, R.J. and Wilt, F. and Davidson, E.H. (1986) Dev. Biol. and submitted for publication.Google Scholar
  30. 30.
    McMahon, A.P., Flytzanis, C.N., Hough-Evans, B.R., Katula, K.S. and Britten, R.J. and Davidson, E.H. (1985) Dev. Biol. 108, 420–430.CrossRefGoogle Scholar
  31. 31.
    Colin, A.M. (1986) in Methods in Cell Biology (Schroeder, T.E. and ed.) Vol. 27, pp. 395–406, Academic Press, New York, NY.Google Scholar
  32. 32.
    Colin, A.M. and Hille, M.B. (1986) Dev. Biol. 115, 184–192.CrossRefGoogle Scholar
  33. 33.
    Gerhart, S.G. (1983) Ph.D. Thesis, University of Calgary, Calgary, Alberta, Canada.Google Scholar
  34. 34.
    Schatten, G. (1982) Int. Rev. Cytology 79, 59–163.CrossRefGoogle Scholar
  35. 35.
    Franks, R.R. and Hough-Evans, B.R. and Britten, R.J. and Davidson, E.H. (1986) (unpublished data).Google Scholar
  36. 36.
    Vitelli, L. and Kemler, I. and Busslinger, M. and Birnstiel, M.S. (1986) Mol. Cell. Biol, (in press).Google Scholar
  37. 37.
    Rubin, G.M. and Spradling, A.C. (1982) Science 218, 348–353.CrossRefGoogle Scholar
  38. 38.
    Spradling, A.C. and Rubin, G.M. (1982) Science 218, 341–347.CrossRefGoogle Scholar
  39. 39.
    Posakony, J.W. and Scheller, R.H. and Anderson, D.M. and Britten, R.J. and Davidson, E.H. (1981) J. Mol. Biol. 149, 41–67.CrossRefGoogle Scholar
  40. 40.
    Liebermann, D. and Hoffman-Liebermann, B. and Weinthal, J. and Childs, G. and Maxson, R. and Mauron, A. and Cohen, S.N. and Kedes, L. (1983) Nature 306, 342–347.CrossRefGoogle Scholar
  41. 41.
    Orr-Weaver, T.L. and Szostak, J.W. and Rothstein, R.J. (1981) Proc. Nat. Acad. Sci. U.S.A. 78, 6354–6358.CrossRefGoogle Scholar
  42. 42.
    Cooper, A.D. and Crain, W.R., Jr. (1982) Nucl. Acids Res. 10, 4081–4092.CrossRefGoogle Scholar
  43. 43.
    Lee, J.J. and Shott, R.J., Rose, S.J., III, Thomas, T.L., Britten, R.J. and Davidson, E.H. (1984) J. Mol. Biol. 172, 149–176.CrossRefGoogle Scholar
  44. 44.
    Davies, J. and Smith, D.I. (1978) Annu. Rev. Microbiol. 32, 469–518.CrossRefGoogle Scholar
  45. 45.
    Berg, D.E. and Jorgenson, R. and Davies, J. (1978) in Microbiology 1978 (Schlessinger, D., ed.) pp. 13–15, American Society of Microbiology, Washington, DC.Google Scholar
  46. 46.
    McKnight, S.L. (1980) Nucl. Acids. Res. 8, 5949–5964.CrossRefGoogle Scholar
  47. 47.
    Folger, K.R. and Wong, E.A., Wahl, G. and Capecchi, M.R. (1982) Mol. Cell. Biol. 2, 1372–1387.Google Scholar
  48. 48.
    Breitman, M.L. and Tsui, L.-C., Buchwald, M. and Siminovitch, L. (1982) Mol. Cell. Biol. 2, 966–976.Google Scholar
  49. 49.
    Miller, C.K. and Temin, H.M. (1983) Science 220, 604–609.CrossRefGoogle Scholar
  50. 50.
    Rusconi, S. and Schaffner, W. (1981) Proc. Nat. Acad. Sci. U.S.A. 78, 5051–5055.CrossRefGoogle Scholar
  51. 51.
    Bendig, M.M. (1981) Nature 292, 65–67.CrossRefGoogle Scholar
  52. 52.
    Signoret, J. and Thiebaud, P., Zefresne, J. and David, J.C. (1986) Dev. Biol. 116, 8–14.CrossRefGoogle Scholar
  53. 53.
    Katula, K.S. and Hough-Evans, B.R. and Flytzanis, C.N. and McMahon, A.P. and Franks, R.R. and Britten, R.J. and Davidson, E.H. (1985) in Banbury Report 20: Genetic Manipulation of the Mammalian Ovum and early Embryo, pp. 231–241, Cold Spring Harbor Laboratory, Cold Spring Harbor, NY.Google Scholar
  54. 54.
    McMahon, A.P, Novak, T.J., Britten, R.J. and Davidson, E.H. (1984) Proc. Nat. Acad. Sci. U.S.A. 81, 7490–7494.CrossRefGoogle Scholar
  55. 55.
    DiNocera, P.P. and Dawid, I.B. (1983) Proc. Nat. Acad. Sci. U.S.A. 80, 7075–7098.CrossRefGoogle Scholar
  56. 56.
    Corces, V. and Pellicer, A., Axel, R. and Meselson, M. (1981) Proc. Nat. Acad. Sci. U.S.A. 78, 7038–7042.CrossRefGoogle Scholar
  57. 57.
    Katula, K.S. and Hough-Evans, B.R. and Britten, R.J. and Davidson, E.H. (1987) Dev. Biol, submitted for publication.Google Scholar
  58. 58.
    Flytzanis, C.N. and Britten, R.J. and Davidson, E.H. (1987) Proc. Nat. Acad. Sci. U.S.A. (in press).Google Scholar
  59. 59.
    Davidson, E.H. and Flytzanis, C.N., Lee, J.J., Robinson, J.J., Rose, S.J. III and Sucov, H.M. (1985) Cold Spring Harbor Symp. Quant. Biol. 50, 321–328.CrossRefGoogle Scholar
  60. 60.
    Mauron, A. and Kedes, L., Hough-Evans, B.R. and Davidson, E.H. (1982) Dev. Biol. 94, 425–434.CrossRefGoogle Scholar
  61. 61.
    Hough-Evans, B.R. and Franks, R.R. and Cameron, R.A. and Britten, R.J. and Davidson, E.H. (1987) Dev. Biol, submitted for publication.Google Scholar
  62. 62.
    Laski, F.A., Rio, D.C. and Rubin, G.A. (1986) Cell 44, 7–19.CrossRefGoogle Scholar
  63. 63.
    Akhurst, R.J. and Calzone, F.J. and Lee, J.J. and Britten, R.J. and Davidson, E.H. (1986) J. Mol. Biol, (in press).Google Scholar

Copyright information

© Plenum Press, New York 1987

Authors and Affiliations

  • Barbara R. Hough-Evans
    • 1
  • Eric H. Davidson
    • 1
  1. 1.Division of BiologyCalifornia Institute of TechnologyPasadenaUSA

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