Escherichia coli Threonyl-Transfer RNA Synthetase as a Model System to Study Translational Autoregulation in Prokaryotes
For many years, it was believed that the regulation of gene expression in prokaryotes occurs only at the transcriptional level. However, many results in the last ten years demonstrate that the process of translation is an important step for the regulation of gene expression and can affect the termination of transcription through polarity or attenuation of transcription. The translation step itself can also be regulated. The first example of translational regulation was found in RNA phages 1 and is still being studied.2 Other examples of genes regulated at the translational level have been discovered, particularly in the case of genes that code for proteins that bind nucleic acids. Examples of these include gene 32 of phage T4,3 the ribosomal protein operons,4 the initiation factor 3 (IF3) gene,5 and the threonyl-transfer RNA (tRNA) synthetase reported herein.
KeywordsLoop Structure Translational Operator Translation Initiation Site Host Integration Factor Anticodon Loop
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