Bacterial Phosphodiesterases (Phospholipases C and D)
The bacterial phosphodiesterases differ from the acyl hydrolase covered in Chapter 2 in that many enzymes of this class are secreted from the cell in a soluble form, a characteristic that has facilitated their purification and characterization. In general, these enzymes attack cellular membranes and either directly or indirectly are involved in cell killing and tissue damage in host organism. Because of their involvement in pathogenesis, they were often identified initially as a toxin before their catalytic function was defined. Indeed, much of the initial interest in bacterial phospholipases was generated by investigators seeking to minimize the effects of bacterial infections.
KeywordsCulture Filtrate Phosphatidic Acid Hemolytic Activity Broad Substrate Specificity Guanidinium Chloride
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