Flow Cytometry: A New Approach to the Isolation and Characterization of Kupffer Cells
Kupffer cells clear portal blood of absorbed proteases, endotoxins and other noxious substances. These cells are well positioned to do so, they reside in the sinusoids of the liver and lie on the luminal side of the sinusoidal endothelium with cytoplasmic processes which insert focally between endothelial cells. Numerically, Kupffer cells comprise approximately 10% of all liver cells (1). Despite their relative abundance, it has been difficult to obtain pure suspensions of Kupffer cells. Cell suspensions containing 40–85% Kupffer cells have been obtained by treating the rat liver in situ with collagenase and subjecting the resulting cell suspension to centrifugal elutriation.
KeywordsKupffer Cell Result Cell Suspension Centrifugal Elutriation Sinusoidal Endothelium Peroxidase Staining
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