Delayed Recovery of Orally Induced Tolerance to Proteins in Irradiated and Spleen-Cell Reconstituted Mice

  • D. G. Hanson
  • T. Morimoto
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 216 A)


A single intragastric (i.g.) feeding of 20 mg of ovalbumin (OVA) or human gammaglobulin (HGG) to normal adult BDF1 mice produces specific tolerance to later parenteral immunization with the antigen. Previous work showed that the tolerance induced by feeding could subsequently be abolished by lethal whole-body irradiation. OVA-specific antibody responses were indistinguishable in normal and orally-tolerized mice that were irradiated and then injected with normal spleen cells immediately prior to immunization (1). In the present study, we examined the effects of irradiation and “reconstitution” with normal spleen cells on the induction of tolerance by OVA and HGG feeding.


Tolerance Induction Villous Height Parenteral Immunization Reconstituted Mouse Farr Assay 
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Copyright information

© Plenum Press, New York 1987

Authors and Affiliations

  • D. G. Hanson
    • 1
  • T. Morimoto
    • 2
  1. 1.Department of Pediatrics, Harvard Medical School and Combined Program in Pediatric Gastroenterology and NutritionMassachusetts General HospitalBostonUSA
  2. 2.Department of MedicineToneyama National HospitalOsakaJapan

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