Delayed Recovery of Orally Induced Tolerance to Proteins in Irradiated and Spleen-Cell Reconstituted Mice
A single intragastric (i.g.) feeding of 20 mg of ovalbumin (OVA) or human gammaglobulin (HGG) to normal adult BDF1 mice produces specific tolerance to later parenteral immunization with the antigen. Previous work showed that the tolerance induced by feeding could subsequently be abolished by lethal whole-body irradiation. OVA-specific antibody responses were indistinguishable in normal and orally-tolerized mice that were irradiated and then injected with normal spleen cells immediately prior to immunization (1). In the present study, we examined the effects of irradiation and “reconstitution” with normal spleen cells on the induction of tolerance by OVA and HGG feeding.
KeywordsTolerance Induction Villous Height Parenteral Immunization Reconstituted Mouse Farr Assay
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- 6.Bruce, M.G., Strobel, S., Hanson, D.G. and Ferguson, A., Intestinal antigen processing is eliminated following irradiation and is restored by normal spleen cells. (In preparation for publication)Google Scholar