Attempts to Enhance RNA Synthesis and Isolate mRNA from Mast Cells
With the development of isolation and purification techniques for mast cells from connective and mucosal tissues, histochemically, biochemically and functionally distinct mast cell subpopulations have been identified (1–6). However, there is little knowledge about the basis of mast cell heterogeneity. It could be due to differences in the stages of differentiation and development, or to microenvironmental influences which induce unique phenotypic expression. Moreover, histochemical and biochemical studies on peritoneal mast cells (PMC) in rats have shown that PMC are, themselves, heterogeneous populations (7,8), a problem which requires careful assessment of the use of the terra “heterogeneity.”
KeywordsMast Cell Interface Fraction Percoll Gradient Peritoneal Mast Cell Pellet Fraction
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