Cofactor Requirements for Expression of Lactoferrin Bactericidal Activity on Enteric Bacteria

  • M. A. Motley
  • R. R. Arnold
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 216 A)


Lactoferrin (LF), is an iron-binding glycoprotein that is common to most mammalian excretions (1–3) and is a prominent component of the specific granule of neutrophilic leukocytes (4,5). It shares its distribution on mucosal surfaces with that of secretory IgA. Its high affinity binding in coordination with suitable anion has been well characterized (6–9). This ability to bind iron has been associated with a bacteriostatic deprivation of this essential nutrient (10–12). A variety of other biological activities have also been attributed to LF, including a direct bactericidal effect on a variety of bacteria (13,14). This killing mechanism is temperature and pH dependent and requires direct interaction of the LF with the bacterial cell surface (15). There are a variety of bacteria that are resistant to the bactericidal effects of purified LF binding including selected Gramnegative enteric bacteria (14). The resistance of these bacteria appears related, in part, to the impermeability of their outer cellular structures (16).


Bactericidal Activity Void Volume Enteric Bacterium Bacteriostatic Activity Human Colostrum 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Masson, D.Y. and Taylor, C.R., J. Clin. Pathol. 31, 316, 1978.CrossRefGoogle Scholar
  2. 2.
    Masson, P.L. and Heremans, J.F., Prot. Biol. Fluids 14, 115, 1966.Google Scholar
  3. 3.
    Masson, P.L., Heremans, J.F. and Dive, C.H., Clin. Chem. Acta. 14, 735, 1966.CrossRefGoogle Scholar
  4. 4.
    Leffell, M. and Spitznagel, J., Infect. Immun. 6, 761, 1972.PubMedGoogle Scholar
  5. 5.
    Masson, P.L., Heremans, J.F. and Schonne, T.Е., J. Exp. Med. 130, 643, 1969.PubMedCrossRefGoogle Scholar
  6. 6.
    Aisen, P., Aasa, R., Malmstrom, B.G. and Vanngard, T., J. Biol. Chem. 242, 2484, 1967.PubMedGoogle Scholar
  7. 7.
    Schade, A.L., Reinhart, R.W. and Levy, H., Arch. Biochem. 20, 170, 1949.PubMedGoogle Scholar
  8. 8.
    Aisen, P. and Leibman, A., Biochim. Biophys. Acta. 304, 797, 1973.PubMedCrossRefGoogle Scholar
  9. 9.
    Bates, G.W. and Schlabach, M.R., FEBS Lett. 33, 289, 1973.PubMedCrossRefGoogle Scholar
  10. 10.
    Oram, J.D. and Reiter, B., Biochim. Biophys. Acta. 170, 351, 1968.PubMedCrossRefGoogle Scholar
  11. 11.
    Weinberg, E., Science 184, 952, 1974.PubMedCrossRefGoogle Scholar
  12. 12.
    Bullen, J.J., Rogers, H.J. and Griffiths, E., Curr. Top. Microbiol. Immunol. 80, 1, 1978.PubMedCrossRefGoogle Scholar
  13. 13.
    Arnold, R., Cole, M. and McGhee, J.R., Science 197, 263, 1977.PubMedCrossRefGoogle Scholar
  14. 14.
    Arnold, R., Brewer, M. and Gauthier, J., Infect. Immun. 28, 893, 1980.PubMedGoogle Scholar
  15. 15.
    Arnold, R., Russell, J., Champion, W. and Gauthier, J., Infect. Immun. 32, 655, 1981.PubMedGoogle Scholar
  16. 16.
    Arnold, R., Russell, J., Champion, W., Brewer, M. and Gauthier, J., Infect. Immun. 35, 792, 1982.PubMedGoogle Scholar
  17. 17.
    Tenovuo, J., Moldoveanu, Z., Mestecky, J., Pruitt, K.M., and Rahemtulla, B.-M., J. Immunol. 128, 726, 1982.PubMedGoogle Scholar
  18. 18.
    Adinolfi, M., Glynn, A.A., Lindsay, M. and Milne, CM., Immunology 10, 517, 1966.PubMedGoogle Scholar
  19. 19.
    Martinez, R.J. and Carroll, S.F., Infect. Immun. 28, 735, 1980.PubMedGoogle Scholar
  20. 20.
    Bullen, J., Rogers, H. and Leigh, L., Brit. Med. J. 1, 69, 1972.PubMedCrossRefGoogle Scholar
  21. 21.
    Rogers, H. and Synge, C., Immunology 34, 19, 1978.PubMedGoogle Scholar
  22. 22.
    Spik, G., Cheron, A., Montreuil, J. and Dolby, J., Immunology 35, 663, 1978.PubMedGoogle Scholar
  23. 23.
    Stephens, S., Dolby, J., Montreuil, J. and Spik, G., Immunology 41, 597, 1980.PubMedGoogle Scholar
  24. 24.
    Arnold, R.R., Russell, J.E., Devine, S.M., Adamson, M. and Pruitt, K.M., in Cariology Today, (Edited by Guggenheim, B.), pp. 75–88, Basel, Karger Press, 1984.Google Scholar
  25. 25.
    Blackberg, L. and Hernell, O., FEBS Lett. 109, 180, 1980.PubMedCrossRefGoogle Scholar
  26. 26.
    Smit, J., Kamio, Y. and Nikaido, H., J. Bacteriol. 124, 942, 1975.PubMedGoogle Scholar
  27. 27.
    Bortner, CA., Miller, R.D. and Arnold, R.R., Infect. Immun. 51, 373, 1986.PubMedGoogle Scholar
  28. 28.
    Dolby, J. and Honour, P., J. Hgy. 83, 255, 1979.CrossRefGoogle Scholar
  29. 29.
    Fitzgerald, S. and Rogers, H., Infect. Immun. 27, 302, 1980.PubMedGoogle Scholar
  30. 30.
    Bullen, J., Cushnie, G. and Rogers, H., Immunology 12, 303, 1967.PubMedGoogle Scholar
  31. 31.
    Bullen, J. and Rogers, H., Nature 224, 380, 1969.PubMedCrossRefGoogle Scholar
  32. 32.
    Bullen, J., Rogers, H. and Lewin, J., Immunology 20, 391, 1971.PubMedGoogle Scholar
  33. 33.
    Rogers, H., Infect. Immun. 7, 445, 1967.Google Scholar

Copyright information

© Plenum Press, New York 1987

Authors and Affiliations

  • M. A. Motley
    • 1
  • R. R. Arnold
    • 2
  1. 1.Department of Microbiology and ImmunologyMorehouse School of MedicineAtlantaUSA
  2. 2.Department of Oral BiologyEmory University Dental Research CenterAtlantaUSA

Personalised recommendations