Presence of Fc Receptors for IgA on Rat Alveolar Macrophages but not Peritoneal Macrophages
Different blood cells, including lymphocytes, polymorphonuclear leukocytes and monocytes, possess a receptor for the Fc portion of IgA (FcaR) on their surface (1–4). Recently, an FcaR has also been demonstrated on mouse alveolar marcrophages (AM) but not peritoneal macrophages (PM) (5), and the IgA-mediated phagocytosis correlated with the presence of FcaR (6). Despite early studies reporting the absence of FcaR on rat and human AM (7,8), up to 17% of human AM can ingest sIgA-opsonized Pseudomonas (9). Further, a possible role of AM has been suggested in a rat model of IgA-induced lung injury (10). Therefore, the present study was designed to assess by flow cytometry the issue of FcaR on rat AM and PM and to investigate whether the molecular size of IgA could influence its binding to the cells.
KeywordsPeritoneal Macrophage Peritoneal Lavage Peritoneal Lavage Fluid Bovine Serum Albumin Control Alveolar Line Fluid
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