Isolation and Characterization of Rabbit Ileal Lamina Propria Mononuclear Cells

  • C. A. Hooper
  • R. H. Reid
  • T. G. Brewer
  • W. T. McCarthy
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 216 A)

Abstract

Most antigens enter the host across a mucosal surface. Admitting nutrients, the surface that has the function of molecule uptake is the gastrointestinal alimentary canal. As molecules cross the mucosal surface of the gut, the molecules encounter the gut associated lymphoid tissue (GALT). Here antigens encounter either a primary or a secondary immune response. Antigen sampling takes place in the Peyer’s patch, some antigens contact the intraepithelial lymphocytes, and the majority of the antigens first encounter immunocytes in the ileal lamina propria (LP) lymphoid cell layer.

Keywords

EDTA Sedimentation Ileal Collagenase 

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References

  1. 1.
    Befus, A.D. and Bienenstock, J., in Animal Models of Immunological Processes (Edited by Hay, J.B.), p. 167, Academic Press, N.Y., 1982.Google Scholar
  2. 2.
    Sell, S., Raffel, C. and Scott, C.B., Developmental and Comparative Immunol. 4, 355, 1980.CrossRefGoogle Scholar
  3. 3.
    Friedenstein, A. and Goncharenko, I., Nature 206, 1113, 1965.PubMedCrossRefGoogle Scholar
  4. 4.
    Cebra, J.J., Craig, S.W. and Jones, P.P., Adv. Exp. Med. Biol. 45, 23, 1974.PubMedGoogle Scholar
  5. 5.
    Crandall, R.B., Cebra, J.J. and Crandall, C.A., Immunology 12, 147, 1967.PubMedGoogle Scholar
  6. 6.
    Crabbe, P.A., Carbonara, A.O. and Heremans, J.F., Lab. Invest. 14, 235, 1965.PubMedGoogle Scholar
  7. 7.
    Bull, D.M. and Bookman, M.A., J. Clin. Invest. 59, 966, 1977.PubMedCrossRefGoogle Scholar
  8. 8.
    McNicholas, J.M., Raffeld, M., Loken, M.R., Reiter, H. and Knight, K.L., Mol. Immunol. 18, 815, 1981.PubMedCrossRefGoogle Scholar
  9. 9.
    Morson, B.C. and Dawson, I.M.P., in Gastrointestinal Pathology, 2nd Ed., p. 219, Blackwell Scientific Publications, Oxford, 1979.Google Scholar
  10. 10.
    Rudzik, O. and Bienenstock, J., Lab. Invest. 30, 260, 1974.PubMedGoogle Scholar
  11. 11.
    Rudzik, O., Clancy, R.L., Perey, D.Y.E., Bienenstock, J. and Singal, D.P., J. Immunol. 114, 1, 1975.PubMedGoogle Scholar
  12. 12.
    Bienenstock, J., Johnston, N. and Perey, D.Y.E., Lab. Invest. 28, 693, 1973.PubMedGoogle Scholar

Copyright information

© Plenum Press, New York 1987

Authors and Affiliations

  • C. A. Hooper
    • 1
  • R. H. Reid
    • 1
  • T. G. Brewer
    • 1
  • W. T. McCarthy
    • 1
  1. 1.Department of GastroenterologyWalter Reed Army Institute of ResearchUSA

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