Murine Peyer’s Patches are Capable of Generating a Cytotoxic T Cell (CTL) Response to Nominal Antigens

  • S. D. London
  • D. H. Rubin
  • J. J. Cebra
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 216 A)


The experiments described in this report were designed to determine whether murine Peyer’s patches are capable of generating a cytotoxic T cell (CTL) response to nominal (non-MHC encoded) antigens. To date, Kagnoff (1) is the only researcher who has been able to directly demonstrate CTL activity in Peyer’s patches. He demonstrated that a cytotoxic T cell response could be detected in Peyer’s patches from mice chronically fed tumor cells that were mismatched at the major histocompatibility locus. However, he was unable to demonstrate a similar response in Peyer’s patches when mice were chronically fed tumor cells bearing minor histocompatibility differences. While his study demonstrated that a CTL response could occur in Peyer’s patches, the generation of allospecific CTL’s, whose precursors are present at high frequency among normal T cells (2), may not be representative of the potential of Peyer’s patches to generate CTL activity directed to nominal antigens. We have utilized reovirus serotype 1 (reo 1) as a model nominal antigen to determine whether intraduodenally applied virus induces a detectable CTL response in murine Peyer’s patches.


Peripheral Lymph Node Peritoneal Exudate Cell Nominal Antigen Reovirus Infection Major Histocompatibility Locus 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Kagnoff, M.F., J. Immunol. 120, 395, 1978.PubMedGoogle Scholar
  2. 2.
    Lindahl, K.F. and Wilson, D.B., J. Exp. Med. 145, 500, 1977.PubMedCrossRefGoogle Scholar
  3. 3.
    Rubin, D.H. and Fields, B.N., J. Exp. Med. 152, 853, 1980.PubMedCrossRefGoogle Scholar
  4. 4.
    Finberg, R., Weiner, H.L., Fields, B.N., Benacerraf, B. and Burakoff, S.J., Proc. Natl. Acad. Sci. USA 76, 442, 1979.PubMedCrossRefGoogle Scholar
  5. 5.
    Wolf, J.L., Rubin, D.H., Finberg, R., Kauffman, R.S., Sharpe, A.H., Trier, J.S. and Fields, B.N., Science 212, 471, 1981.PubMedCrossRefGoogle Scholar
  6. 6.
    Fazekas De St. Groth, S., J. Immunol. Meth. 49, R11, 1982.CrossRefGoogle Scholar
  7. 7.
    Finberg, R., Spriggs, D.R. and Fields, B.N., J. Immunol. 129, 2235, 1982.PubMedGoogle Scholar
  8. 8.
    Tagliabue, A., Villa, L., Scapigliati, G. and Boraschi, D., Nat. Immun. Cell Growth Regul. 3, 95, 1983/1984.Google Scholar
  9. 9.
    Kiyono, H., McGhee, J.R., Wannemuehler, M.J., Frangakis, M.V., Spalding, D.M., Michalek, S.M. and Koopman, W.J., Proc. Natl. Acad. Sci. USA 79, 596, 1982.PubMedCrossRefGoogle Scholar
  10. 10.
    Reynolds, J., Dudler, H.L. and Trnka, Z., Immunology 47, 415, 1982.PubMedGoogle Scholar
  11. 11.
    McDermott, M.R., Horsewood, P., Clark, D.A. and Bienenstock, J., Immunology 57, 213, 1986.PubMedGoogle Scholar
  12. 12.
    Gallatin, W.M., Weissman, I.L. and Butcher, E.C., Nature 304, 30, 1983.PubMedCrossRefGoogle Scholar
  13. 13.
    Fields, B.N., Arch. Virol. 71, 95, 1982.PubMedCrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1987

Authors and Affiliations

  • S. D. London
    • 1
    • 2
  • D. H. Rubin
    • 1
    • 2
  • J. J. Cebra
    • 1
    • 2
  1. 1.Department of BiologyUniversity of PennsylvaniaPhiladelphiaUSA
  2. 2.Departments of Medicine and Microbiology, Veterans Administration Medical CenterUniversity of PennsylvaniaPhiladelphiaUSA

Personalised recommendations