A Novel Pre-B Cell Precursor: Phenotypic Characterization and Differentiation Induction by Dendritic Cell-T Cell Mixtures

  • D. M. Spalding
  • J. A. Griffin
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 216 A)


Recently, culture systems have been developed which support the growth of multipotential stem cells (1), pro-B cells (2), pre-B cells (3) or mixtures of several early stages (4,5), and these have begun to improve our understanding of very early stages of B lymphocyte differentiation. In some of these systems, differentiation to a subsequent developmental stage can be induced, but multiple levels of differentiation have not been observed under conditions suitable for detailed analysis of phenotypic and genotypic events during these maturation processes.


Cytoplasmic Staining Cell Stage Multipotential Stem Cell Cytocentrifuge Preparation Subsequent Developmental Stage 
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Copyright information

© Plenum Press, New York 1987

Authors and Affiliations

  • D. M. Spalding
    • 1
    • 2
  • J. A. Griffin
    • 1
    • 2
  1. 1.Division of Clinical Immunology and Rheumatology, and Departments of Medicine, Microbiology and BiochemistryUniversity of Alabama at BirminghamBirminghamUSA
  2. 2.the Birmingham Veterans Administration HospitalBirminghamUSA

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