A Predominant Idiotype in the Gut Associated Lymphoid Tissue
A high percentage of gut lamina propria plasma cells in tissue sections were stained by three fluoresceinated rabbit anti mouse IgG3 antisera. Similar cytoplasmic staining was found in several mouse strains including CBA/N mice. To further extend these data, northern hybridization experiments with a cDNA probe specific for IgG3 was performed with total RNA isolated from the small intestine or from cell preparations enriched for lamina propria plasma cells (data not shown). In contrast to the fluorescent studies, little or no mRNA for IgG3 was found. In all cases the antigen used in producing the anti-mouse IgG3 serum was the J 606 (IgG3) myeloma protein, which reacts with certain polysaccharides such as inulin [β(2–1) poly fructosan] and bacterial levan [β(2–6) polyfructosan].
KeywordsPlasma Cell Antigen Binding Site Myeloma Protein Conjugate Goat Anti Rabbit Idiotypic Determinant
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