Regulatory T Cells in the Galt
The discussion about the importance of regulatory T cells for the differentiation of B cell precursors into IgA producing cells is controversial. Whereas the antigen driven differentiation of precursor cells is the central point of the B cell centered theories (1), others have suggested a crucial influence of T cells for the end stage differentiation of bone marrow derived cells into IgA secreting cells (2). Our own experiments with PP-deprived rats and mice show that these animals have lower IgA-positive cells in the lamina propria (LP) of the small intestine. This result points to the fact that the antigenic load, which due to gut motility should induce a more intense contact with the GALT, was not the ultimate inducer for an IgA response in the intestine. These findings made us consider the role of regulatory T cells for the IgA secretion in the LP. As a source of T cells we used phytahemagglutinin (PHA)-driven PP T cells and investigated their influence on bone marrow cells and as a source of precursor B cells PNA separated bone marrow cells. In another experiment we looked for the role of PHA directly on unseparated Peyer’s patch, spleen and LP cells.
KeywordsBone Marrow Cell Lamina Propria Spleen Cell Lamina Propria Lymphocyte Lamina Propria Cell
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- 2.Strober, W. and Jacobs, D., in Advances in Host Defense Mechanisms, (Edited by Gallin, J.I. and Fauci, A.S.), p. 1, Raven Press, 1985.Google Scholar