Isolation and Enumeration of Isotype Specific Plaque Forming Cells from the Murine Intestine to Study the Development of the Intestinal B Cell Background Response

  • A. T. J. Bianchi
  • Ph. J. van der Heijden
  • W. Stok
  • J. W. Scholten
  • B. A. Bokhout
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 216 A)


Quantification of (specific) IgA in faeces using the ELISA technique (1,2) and localization of antibody containing cells (ACC) by immunohistology (3,4) are important methods to investigate the in vivo expression of the enteric humoral immune response. These methods have improved our knowledge of the expression of the mucosal immune system, but they have several restrictions. Immunohistological studies give a direct insight in the in vivo expression of the mucosal immune system itself, but quantitative data are very difficult to obtain and the number of ACC does not reflect the number of B cells secreting antibodies (5) contributing to the intestinal IgA response. Moreover, IgA measured in faeces is not merely a reflection of locally (intestinally) produced and secreted IgA, since systemically produced IgA can also contribute to the IgA levels in faeces (6). Knowledge about the quantitative expression of the enteric humoral immune response can be obtained by isolation of lymphocytes from the intestine which can be tested subsequently for their capacities to secrete (antigen-specific) IgA.


Lamina Propria Isolation Procedure Mucosal Immune System Antibody Secreting Cell Lamina Propria Lymphocyte 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Elson, C.O., Ealding, W. and Lefkowitz, J.J., Immunol. Meth. 67, 101, 1984.CrossRefGoogle Scholar
  2. 2.
    Zaane, van D. and I Jzerman, J., Immunol. Meth. 72, 427, 1984.CrossRefGoogle Scholar
  3. 3.
    Sminia, T., Delemarre, F. and Janse, E.M., Immunology 50, 53, 1983.PubMedGoogle Scholar
  4. 4.
    Pierce, N.F., Infect. Immun. 43, 341, 1984.PubMedGoogle Scholar
  5. 5.
    Benner, R., van Oudenaren, A., Björklund, M., Ivars, F. and Holmberg, D., Immunol. Today 3, 243, 1982.CrossRefGoogle Scholar
  6. 6.
    Solari, R. and Kraehenbuhl, J.P., Immunol. Today 6, 17, 1985.CrossRefGoogle Scholar
  7. 7.
    Bull, D.M. and Bookman, M.A., J. Clin. Invest. 59, 966, 1977.PubMedCrossRefGoogle Scholar
  8. 8.
    Bartnik, W., Remine, S.G., Cluba, M., Thayer, W.R. and Shorter, R.G., Gastroenterology 76, 976, 1980.Google Scholar
  9. 9.
    Rudzik, O. and Bienenstock, J., Lab. Invest. 30, 260, 1974.PubMedGoogle Scholar
  10. 10.
    Watson, D.L., Bennell, M.A. and Chaniago, T.D., Am. J. Vet. Res. 40, 61, 1979.PubMedGoogle Scholar
  11. 11.
    Lyscom, N. and Brueton, M.J., Immunology 45, 775, 1982.PubMedGoogle Scholar
  12. 12.
    Davies, M.D.J, and Parrott, D.M.V., Gut 22, 481, 1981.PubMedCrossRefGoogle Scholar
  13. 13.
    Tseng, J., Cell. Immunol. 73, 324, 1982.PubMedCrossRefGoogle Scholar
  14. 14.
    van der Heijden, P.J., Bianchi, A.T.J., Oenema, R.G., Scholten, J.W., Stok, W. and Swart, R.J., Proc. 4th Int. Symp. Vet. Lab. Diagnosticans 745, 1986.Google Scholar
  15. 15.
    van der Heijden, P.J., Bokhout, B.A., Bianchi, A.T.J., Scholten, J.W. and Stok, W., Immunobiology 171, 143, 1986.PubMedCrossRefGoogle Scholar
  16. 16.
    Andre, C., Andre, F., Druget, M. and Fargier, M.C., Adv. Exp. Med. Biol. 107, 583, 1978.PubMedCrossRefGoogle Scholar
  17. 17.
    Cebra, J.J., Gearhart, P.J., Kamat, R., Robertson, S.M. and Tseng, J., Abstracts of Communications, BSI Meeting 5, 1565, 1977.Google Scholar
  18. 18.
    Ernst, P.B., Befus, A.B. and Bienenstock, J., Immunol. Today 6, 50, 1985.CrossRefGoogle Scholar
  19. 19.
    Dillon, S.B. and Macdonald, T.T., Immunology 52, 501, 1984.PubMedGoogle Scholar
  20. 20.
    Tagliabue, A., Befus, A.D., Clark, D.A. and Bienenstock, J., J. Exp. Med. 155, 1785, 1982.PubMedCrossRefGoogle Scholar
  21. 21.
    Benner, R., Rijnbeek, A.M., Bernabè, R.R., Matinez-Alonso, C. and Coutinho, A., Immunobiology 158, 225, 1981.PubMedCrossRefGoogle Scholar
  22. 22.
    Benner, R.,N The Immune System, (Edited by Steinberg and Lefkovits), S. Karger, Basel, 1981.Google Scholar

Copyright information

© Plenum Press, New York 1987

Authors and Affiliations

  • A. T. J. Bianchi
    • 1
  • Ph. J. van der Heijden
    • 1
  • W. Stok
    • 1
  • J. W. Scholten
    • 1
  • B. A. Bokhout
    • 1
  1. 1.Dept. of ImmunologyCentral Veterinary InstituteLelystadThe Netherlands

Personalised recommendations