A Microcomputer-Based System for the Recording and Analysis of Fluorescence Transients from Membrane Vesicles
Until a few years ago, the problem of studying the kinetics of transport at the tonoplast seemed almost insurmountable. The only technique available was that of compartmental analysis, which, as a prerequisite for its successful application, necessitated that the cell be at steady state with respect of solute concentration. Thus, although unidirectional fluxes could be derived from the analysis of isotopic efflux, dynamic studies were precluded.
KeywordsAcridine Orange Fluorescence Spectrometer Fluorescence Transient Plant Vacuole Unidirectional Flux
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