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A Microcomputer-Based System for the Recording and Analysis of Fluorescence Transients from Membrane Vesicles

  • Ian R. Jennings
  • Philip A. Rea
  • Dale Sanders
Part of the NATO ASI Series book series (NSSA, volume 134)

Abstract

Until a few years ago, the problem of studying the kinetics of transport at the tonoplast seemed almost insurmountable. The only technique available was that of compartmental analysis, which, as a prerequisite for its successful application, necessitated that the cell be at steady state with respect of solute concentration. Thus, although unidirectional fluxes could be derived from the analysis of isotopic efflux, dynamic studies were precluded.

Keywords

Acridine Orange Fluorescence Spectrometer Fluorescence Transient Plant Vacuole Unidirectional Flux 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Blumwald, E., Rea, P. A., and Poole, R. J., 1987, Preparation of tonoplast vesicles: Applications to H+-coupled secondary transport in plant vacuoles, Methods In Enzymology, in press.Google Scholar
  2. Marquardt, D., 1963, An algorithm for least squares estimation of non-linear parameters, J. Soc. Industr. Appl. Math., 11: 431.Google Scholar

Copyright information

© Plenum Press, New York 1987

Authors and Affiliations

  • Ian R. Jennings
    • 1
  • Philip A. Rea
    • 1
  • Dale Sanders
    • 1
  1. 1.Department of BiologyUniversity of YorkHeslington, YorkEngland, UK

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