Influence of External pH on Alkaloid Production and Excretion by Catharanthus Roseus Resting Cell Suspensions
Catharanthus roseus (L.) G. Don, cell line C20, cultivated in a batch culture system with a modified Gamborg’s B5 medium (Gamborg et al., 1968) deprived in auxin (2,4-dichlorophenoxy-acetic acid) and enriched in mannitol (200 mM) were maintained in survival conditions during 10 days after the growth phase (Ambid et al., 1982). The production of two alkaloids: ajmalicine and serpentine, was then largely emphazised (Roustan et al., 1982) and these compounds were essentially located within the cells.
KeywordsIndole Alkaloid Alkaloid Production Batch Condition Catharanthus Roseus Alkaloid Extraction
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