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Plant Vacuoles pp 529-533 | Cite as

Influence of External pH on Alkaloid Production and Excretion by Catharanthus Roseus Resting Cell Suspensions

  • Claudine Nef
  • Christian Ambid
  • Jean Fallot
Part of the NATO ASI Series book series (NSSA, volume 134)

Abstract

Catharanthus roseus (L.) G. Don, cell line C20, cultivated in a batch culture system with a modified Gamborg’s B5 medium (Gamborg et al., 1968) deprived in auxin (2,4-dichlorophenoxy-acetic acid) and enriched in mannitol (200 mM) were maintained in survival conditions during 10 days after the growth phase (Ambid et al., 1982). The production of two alkaloids: ajmalicine and serpentine, was then largely emphazised (Roustan et al., 1982) and these compounds were essentially located within the cells.

Keywords

Indole Alkaloid Alkaloid Production Batch Condition Catharanthus Roseus Alkaloid Extraction 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Ambid, C., Roustan, J. P., Nef, C., and Fallot, J., 1982, Influence of 2,4-dichlorophenoxyacetic on the production of ajmalicine and serpentine by cell suspension cultures of Catharanthus roseus, in “Proceedings of the Fifth International Congress of Plant Tissue and Cell CultureGoogle Scholar
  2. Deus-Neumann, B., and Zenk, M. H., 1986, Accumulation of alkaloids in plant vacuoles does not involve an ion-trap mechanism, Planta, 167: 44.CrossRefGoogle Scholar
  3. Gamborg, O. L., Miller, R. A., and Ojima, K., 1968, Nutrient requirement of suspension cultures of soybean root cells, Exp. Cell Res., 50: 151.PubMedCrossRefGoogle Scholar
  4. Renaudin, J. P., and Guern, J., 1982, Compartmentation mechanisms of indole alkaloids in cell suspension cultures of Catharanthus roseus (L.) G. Don, Physiol. Vég., 20: 533.Google Scholar
  5. Roustan, J. P., Ambid, C., and Fallot, J., 1982, Influence de l’acide 2,4-dichlorophénoxyacétique sur l’accumulation de certains alcaloides indoliques dans les cellules quiescentes de Catharanthus roseus cultivées in vitro, Physiol. Vég., 20: 523.Google Scholar

Copyright information

© Plenum Press, New York 1987

Authors and Affiliations

  • Claudine Nef
    • 1
  • Christian Ambid
    • 2
  • Jean Fallot
    • 2
  1. 1.Laboratoire de Physiologie VégétaleORSTOMAbidjanCôte-d’Ivoire
  2. 2.Laboratoire de Biotechnologie VégétaleE. N. S. A. T.Toulouse-CedexFrance

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